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dc.contributor.authorChandran, Sreenath
dc.contributor.authorCairns, Michael T.
dc.contributor.authorO'Brien, Margaret
dc.contributor.authorO'Connell, Enda
dc.contributor.authorMashayekhi, Kaveh
dc.contributor.authorSmith, Terry J.
dc.date.accessioned2016-08-04T09:06:50Z
dc.date.issued2016-01-01
dc.identifier.citationChandran, S,Cairns, MT,O'Brien, M,O'Connell, E,Mashayekhi, K,Smith, TJ (2016) 'Effects of combined progesterone and 17 beta-estradiol treatment on the transcriptome of cultured human myometrial smooth muscle cells'. Physiological Genomics, 48 :50-61.en_IE
dc.identifier.issn1531-2267
dc.identifier.urihttp://hdl.handle.net/10379/5925
dc.descriptionJournal articleen_IE
dc.description.abstractA transcriptomic analysis of cultured human uterine smooth muscle cells (hUtSMCs) was performed to examine gene expression profiles in smooth muscle in an environment containing the two major steroid hormones that regulate the human myometrium in physiological states associated with estrous, pregnancy, labor, and pathophysiological states such as leiomyoma and endometrial cancer. hUtSMCs were treated with progesterone (P4) and 17 beta-estradiol (E2) individually and in combination, in the presence and absence of RU486 (mifepristone). Transcription of many genes was modulated in the presence of P4 or E2 alone, but almost six times more genes were transcriptionally modulated in the presence of the P4/E2 hormone combination. In total 796 annotated genes were significantly differentially expressed in the presence of both P4 and E2 relative to their expression in untreated cells. Functional withdrawal of P4 by addition of RU486 effectively reversed almost all transcriptional changes caused by P4/E2 treatment. Gene ontology analysis of differentially expressed genes revealed a strong association between P4/E2 treatment and downregulated expression of genes involved in cell communication, signal transduction, channel activity, inflammatory response, and differentiation. Upregulated processes included cell survival, gene transcription, steroid hormone biosynthesis, muscle development, insulin receptor signaling, and cell growth.en_IE
dc.description.sponsorshipScience Foundation Irelanden_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherAmerican Physiological Societyen_IE
dc.relation.ispartofPhysiological Genomicsen
dc.subjectProgesteroneen_IE
dc.subject17 Beta-estradiolen_IE
dc.subjectUterine smooth muscle cellsen_IE
dc.subjectMicroarrayen_IE
dc.subjectTranscriptomicsen_IE
dc.subjectPregnant rat myometriumen_IE
dc.subjectTerm human myometriumen_IE
dc.subjectGene expressionen_IE
dc.subjectUp-regulationen_IE
dc.subjectUterine leiomyomaen_IE
dc.subjectOxytocin receptoren_IE
dc.subjectHuman parturitionen_IE
dc.subjectCDNA microarrayen_IE
dc.subjectEstrogenen_IE
dc.subjectProteinen_IE
dc.titleEffects of combined progesterone and 17 beta-estradiol treatment on the transcriptome of cultured human myometrial smooth muscle cellsen_IE
dc.typeArticleen_IE
dc.date.updated2016-07-27T08:11:24Z
dc.identifier.doi10.1152/physiolgenomics.00021.2015
dc.local.publishedsourcehttp://dx.doi.org/10.1152/physiolgenomics.00021.2015
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.description.embargo2017-01-01
dc.internal.rssid10620146
dc.local.contactTerry Smith, School Of Natural Sciences, Nui Galway. 2022 Email: terry.smith@nuigalway.ie
dc.local.copyrightcheckedNo I have forwarded a pre-print of the manuscript, which was accepted for publication. Hopefully that allows its inclusion in the ARAN repository. I have already uploaded this to ResearchGate. Terry Smith
dc.local.versionACCEPTED
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