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dc.contributor.authorUtratna, Marta
dc.contributor.authorCosgrave, Eoin
dc.contributor.authorBaustian, Claas
dc.contributor.authorCeredig, Rhodri H.
dc.contributor.authorO’Byrne, Conor P.
dc.identifier.citationUtratna, M,Cosgrave, E,Baustian, C,Ceredig, RH,O'Byrne, CP (2014) 'Effects of growth phase and temperature on sigma(B) Activity within a Listeria monocytogenes population: evidence for RsbV-independent activation of sigma(B) at refrigeration temperatures' Biomed Research International, .en_IE
dc.identifier.otherArticle ID: 641647
dc.descriptionJournal articleen_IE
dc.description.abstractThe alternative sigma factor sigma(B) of Listeria monocytogenes is responsible for regulating the transcription of many of the genes necessary for adaptation to both food-related stresses and to conditions found within the gastrointestinal tract of the host. The present study sought to investigate the influence of growth phase and temperature on the activation of sigma(B) within populations of L. monocytogenes EGD-e wild-type, Delta sigB, and Delta rsbV throughout growth at both 4 degrees C and 37 degrees C, using a reporter fusion that couples expression of EGFP to the strongly sigma(B)-dependent promoter of lmo2230. A similar sigma(B) activation pattern within the population was observed in wt-egfp at both temperatures, with the highest induction of sigma(B) occurring in the early exponential phase of growth when the fluorescent population rapidly increased, eventually reaching the maximum in early stationary phase. Interestingly, induction of sigma(B) activity was heterogeneous, with only a proportion of the cells in the wt-egfp population being fluorescent above the background autofluorescence level. Moreover, significant RsbV-independent activation of sigma(B) was observed during growth at 4 degrees C. This result suggests that an alternative route to sigma(B) activation exists in the absence of RsbV, a finding that is not explained by the current model for sigma(B) regulation.en_IE
dc.description.sponsorshipScience Foundation Ireland Research Frontiers Programme Grant no. 05/RFP/GEN0044; SFI Grant no. SRC 09/SRC/B1794; SFI Stokes Professorshipen_IE
dc.publisherHindawi Publishing Corporationen_IE
dc.relation.ispartofBiomed Research Internationalen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjectGeneral stress responseen_IE
dc.subjectGram-positive bacteriaen_IE
dc.subjectBacillus subtilisen_IE
dc.subjectTranscription factoren_IE
dc.subjectEnergy stressen_IE
dc.subjectCold growthen_IE
dc.titleEffects of growth phase and temperature on sigma(B) Activity within a Listeria monocytogenes population: evidence for RsbV-independent activation of sigma(B) at refrigeration temperaturesen_IE
dc.description.peer-reviewedPeer reviewed
dc.local.contactRhodri Ceredig, Room 1-1018 Biosciences, Newcastle Road, Dangan, Nui Galway. 5916 Email:

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