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dc.contributor.authorBarron, Valerie
dc.contributor.authorNeary, Martin
dc.contributor.authorMohamed, Khalid Merghani Salid
dc.contributor.authorAnsboro, Sharon
dc.contributor.authorShaw, Georgina
dc.contributor.authorO'Malley, Grace
dc.contributor.authorRooney, Niall
dc.contributor.authorBarry, Frank
dc.contributor.authorMurphy, Mary
dc.date.accessioned2016-06-09T07:37:20Z
dc.date.issued2015-10-05
dc.identifier.citationBarron, V,Neary, M,Mohamed, KMS,Ansboro, S,Shaw, G,O'Malley, G,Rooney, N,Barry, F,Murphy, M (2016) 'Evaluation of the Early In Vivo Response of a Functionally Graded Macroporous Scaffold in an Osteochondral Defect in a Rabbit Model'. Annals Of Biomedical Engineering, 44 :1832-1844.en_IE
dc.identifier.issn1573-9686
dc.identifier.urihttp://hdl.handle.net/10379/5869
dc.description.abstractCartilage tissue engineering is a multifactorial problem requiring a wide range of material property requirements from provision of biological cues to facilitation of mechanical support in load-bearing diarthrodial joints. The study aim was to design, fabricate and characterize a template to promote endogenous cell recruitment for enhanced cartilage repair. A polylactic acid poly-epsilon-caprolactone (PLCL) support structure was fabricated using laser micromachining technology and thermal crimping to create a functionally-graded open pore network scaffold with a compressive modulus of 9.98 +/- A 1.41 MPa and a compressive stress at 50% strain of 8.59 +/- A 1.35 MPa. In parallel, rabbit mesenchymal stem cells were isolated and their growth characteristics, morphology and multipotency confirmed. Sterilization had no effect on construct chemical structure and cellular compatibility was confirmed. After four weeks implantation in an osteochondral defect in a rabbit model to assess biocompatibility, there was no evidence of inflammation or giant cells. Moreover, acellular constructs performed better than cell-seeded constructs with endogenous progenitor cells homing through microtunnels, differentiating to form neo-cartilage and strengthening integration with native tissue. These results suggest, albeit at an early stage of repair, that by modulating the architecture of a macroporous scaffold, pre-seeding with MSCs is not necessary for hyaline cartilage repair.en_IE
dc.description.sponsorshipEuropean Union’s 7th Framework Programme under Grant Agreement No. HEALTH-2007-B-223298 (PurStem), Science Foundation Ireland (Grant Number 09/SRC/B1794), Wellcome Trust Biomedical Vacation Scholarships Grant Number WTD004448 and the Irish Government’s Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007–2013en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherSpringer Verlagen_IE
dc.relation.ispartofAnnals Of Biomedical Engineeringen
dc.subjectFunctionally gradeden_IE
dc.subjectPolylactic acid-e-polycaprolactoneen_IE
dc.subjectCartilage repairen_IE
dc.subjectIntegrationen_IE
dc.subjectNeotissue formationen_IE
dc.subjectMesenchymal stem cellsen_IE
dc.subjectcarten_IE
dc.subjectArticular cartilageen_IE
dc.subjectMechanical propertiesen_IE
dc.subjectTissue formationen_IE
dc.subjectRegenerationen_IE
dc.subjectTherapyen_IE
dc.subjectCarrieren_IE
dc.subjectChondrogenesisen_IE
dc.subjectRegenerative medicineen_IE
dc.titleEvaluation of the early in vivo response of a functionally graded macroporous scaffold in an osteochondral defect in a rabbit modelen_IE
dc.typeArticleen_IE
dc.date.updated2016-06-08T12:53:51Z
dc.identifier.doi10.1007/s10439-015-1473-6
dc.local.publishedsourcehttp://dx.doi.org/10.1007/s10439-015-1473-6en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.description.embargo2016-10-05
dc.internal.rssid11014110
dc.local.contactMary Murphy, Dept. Of Medicine & Remedi, Orbsen Building, Nui, Galway. 5206 Email: mary.murphy@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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