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dc.contributor.authorBarron, Valerie
dc.contributor.authorMerghani, Khalid
dc.contributor.authorShaw, Georgina
dc.contributor.authorColeman, Cynthia
dc.contributor.authorHayes, Jessica
dc.contributor.authorAnsboro, Sharon
dc.contributor.authorManian, Abi
dc.contributor.authorO'Malley, Grace
dc.contributor.authorConnolly, Emma
dc.contributor.authorNandakumar, Ananda
dc.contributor.authorVan Blitterswijk, Clemen
dc.contributor.authorHabibovic, Pamela
dc.contributor.authorMoroni, Lorenzo
dc.contributor.authorShannon, Fintan
dc.contributor.authorBarry, Frank
dc.contributor.authorMurphy, Mary
dc.date.accessioned2016-06-08T13:34:17Z
dc.date.available2016-06-08T13:34:17Z
dc.date.issued2015-01-15
dc.identifier.citationBarron, V,Merghani, K,Shaw, G,Coleman, CM,Hayes, JS,Ansboro, S,Manian, A,O'Malley, G,Connolly, E,Nandakumar, A,van Blitterswijk, CA,Habibovic, P,Moroni, L,Shannon, F,Murphy, JM,Barry, F (2015) 'Evaluation of Cartilage Repair by Mesenchymal Stem Cells Seeded on a PEOT/PBT Scaffold in an Osteochondral Defect'. Annals Of Biomedical Engineering, 43 :2069-2082.en_IE
dc.identifier.issn1573-9686
dc.identifier.urihttp://hdl.handle.net/10379/5868
dc.description.abstractThe main objective of this study was to evaluate the effectiveness of a mesenchymal stem cell (MSC)-seeded polyethylene-oxide-terephthalate/polybutylene-terephthalate (PEOT/PBT) scaffold for cartilage tissue repair in an osteochondral defect using a rabbit model. Material characterisation using scanning electron microscopy indicated that the scaffold had a 3D architecture characteristic of the additive manufacturing fabrication method, with a strut diameter of 296 +/- A 52 mu m and a pore size of 512 +/- A 22 mu m x 476 +/- A 25 mu m x 180 +/- A 30 mu m. In vitro optimisation revealed that the scaffold did not generate an adverse cell response, optimal cell loading conditions were achieved using 50 mu g/ml fibronectin and a cell seeding density of 25 x 10(6) cells/ml and glycosaminoglycan (GAG) accumulation after 28 days culture in the presence of TGF beta 3 indicated positive chondrogenesis. Cell-seeded scaffolds were implanted in osteochondral defects for 12 weeks, with cell-free scaffolds and empty defects employed as controls. On examination of toluidine blue staining for chondrogenesis and GAG accumulation, both the empty defect and the cell-seeded scaffold appeared to promote repair. However, the empty defect and the cell-free scaffold stained positive for collagen type I or fibrocartilage, while the cell-seeded scaffold stained positive for collagen type II indicative of hyaline cartilage and was statistically better than the cell-free scaffold in the blinded histological evaluation. In summary, MSCs in combination with a 3D PEOT/PBT scaffold created a reparative environment for cartilage repair.en_IE
dc.description.sponsorshipScience Foundation Ireland (SFI) Strategic Research Cluster (SRC), Grant No. SFI: 09/SRC B1794, Wellcome Trust Biomedical Vacation Scholarships Grant Number WTD004448, the European Union’s 7th Framework Programme under Grant Agreement No. HEALTH-2007-B- 223298 (PurStem)en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherSpringer Verlagen_IE
dc.relation.ispartofAnnals Of Biomedical Engineeringen
dc.subjectAdditive manufacturingen_IE
dc.subject3D scaffolden_IE
dc.subjectPEOT/PBTen_IE
dc.subjectMesenchymal stem cellsen_IE
dc.subjectCartilage repairen_IE
dc.subjectDynamic mechanical propertiesen_IE
dc.subjectAnimal modelsen_IE
dc.subjectArticular cartilageen_IE
dc.subjectTissueen_IE
dc.subjectRGDen_IE
dc.subjectDifferentiationen_IE
dc.subjectRegenerationen_IE
dc.subjectSocietyen_IE
dc.titleEvaluation of cartilage repair by mesenchymal stem cells seeded on a PEOT/PBT scaffold in an osteochondral defecten_IE
dc.typeArticleen_IE
dc.date.updated2016-06-08T12:51:39Z
dc.identifier.doi10.1007/s10439-015-1246-2
dc.local.publishedsourcehttp://dx.doi.org/10.1007/s10439-015-1246-2en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid9392603
dc.local.contactMary Murphy, Dept. Of Medicine & Remedi, Orbsen Building, Nui, Galway. 5206 Email: mary.murphy@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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