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dc.contributor.authorLeszczynska, Aleksandra
dc.contributor.authorO'Doherty, Aideen
dc.contributor.authorFarrell, Eric
dc.contributor.authorPindjakova, Jana
dc.contributor.authorO'Brien, Fergal J.
dc.contributor.authorO'Brien, Timothy
dc.contributor.authorBarry, Frank
dc.contributor.authorMurphy, Mary
dc.date.accessioned2016-06-08T13:07:03Z
dc.date.available2016-06-08T13:07:03Z
dc.date.issued2016-02-27
dc.identifier.citationLeszczynska A;O'Doherty A;Farrell E;Pindjakova J;O'Brien FJ;O'Brien T;Barry F;Murphy M; (2016) 'Differentiation of Vascular Stem Cells Contributes to Ectopic Calcification of Atherosclerotic Plaque'. Stem Cells (Dayton, Ohio), 34 (4).en_IE
dc.identifier.issn1549-4918
dc.identifier.urihttp://hdl.handle.net/10379/5867
dc.description.abstractThe cellular and molecular basis of vascular calcification (VC) in atherosclerosis is not fully understood. Here, we investigate role of resident/circulating progenitor cells in VC and contribution of inflammatory plaque environment to this process. Vessel-derived stem/progenitor cells (VSCs) and mesenchymal stem cells (MSCs) isolated from atherosclerotic ApoE(-/-) mice showed significantly more in vitro osteogenesis and chondrogenesis than cells generated from control C57BL/6 mice. To assess their ability to form bone in vivo, cells were primed chondrogenically or cultured in control medium on collagen glycosaminoglycan scaffolds in vitro prior to subcutaneous implantation in ApoE(-/-) and C57BL/6 mice using a crossover study design. Atherosclerotic ApoE(-/-) MSCs and VSCs formed bone when implanted in C57BL/6 mice. In ApoE(-/-) mice, these cells generated more mature bone than C57BL/6 cells. The atherosclerotic in vivo environment alone promoted bone formation by implanted C57BL/6 cells. Un-primed C57BL/6 VSCs were unable to form bone in either mouse strain. Treatment of ApoE(-/-) VSC chondrogenic cultures with interleukin (IL)-6 resulted in significantly increased glycosaminoglycan deposition and expression of characteristic chondrogenic genes at 21 days. In conclusion, resident vascular cells from atherosclerotic environment respond to the inflammatory milieu and undergo calcification. IL-6 may have a role in aberrant differentiation of VSCs contributing to vascular calcification in atherosclerosis. Stem Cells 2016;34:913-923.en_IE
dc.description.sponsorshipIrish Research Council for Science, Engineering and Technology and Science Foundation Ireland under Grant No. 09/SRC/B1794en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherWileyen_IE
dc.relation.ispartofStem Cells (Dayton, Ohio)en
dc.subjectVascular progenitor cellsen_IE
dc.subjectMesenchymal stem cellsen_IE
dc.subjectPericytesen_IE
dc.subjectAtherosclerosisen_IE
dc.subjectVascular calcificationen_IE
dc.subjectChondrogenesisen_IE
dc.subjectEndochondral ossificationen_IE
dc.subjectCollagen scaffolden_IE
dc.subjectIn vivoen_IE
dc.titleDifferentiation of vascular stem cells contributes to ectopic calcification of atherosclerotic plaqueen_IE
dc.typeArticleen_IE
dc.date.updated2016-06-08T12:05:14Z
dc.identifier.doi10.1002/stem.2315
dc.local.publishedsourcehttp://dx.doi.org/10.1002/stem.2315en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid11060872
dc.local.contactMary Murphy, Dept. Of Medicine & Remedi, Orbsen Building, Nui, Galway. 5206 Email: mary.murphy@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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