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dc.contributor.authorJohnson, Mark C.
dc.contributor.authorSangrador-Vegas, Amaia
dc.contributor.authorSmith, Terry
dc.contributor.authorCairns, Michael T.
dc.date.accessioned2016-04-13T07:56:02Z
dc.date.available2016-04-13T07:56:02Z
dc.date.issued2006-04-15
dc.identifier.citationJohnson, MC,Sangrador-Vegas, A,Smith, TJ,Cairns, MT (2006) 'Cloning and characterization of two genes encoding rainbow trout homologues of the IFITM protein family'. Veterinary Immunology And Immunopathology, 110 :357-362.en_IE
dc.identifier.issn1873-2534
dc.identifier.urihttp://hdl.handle.net/10379/5668
dc.description.abstractTo identify genes connected with leucocyte function in rainbow trout a cDNA library derived front head kidney leucocytes exposed to phytohemagglutinin (PHA) was screened. A clone with homology to the previously described human interferon-inducible transmembrane proteins (IFITM) was isolated. This clone was used to screen a trout genomic library identifying two trout IFITM genes. Both genes are composed of two exons, in common with human IFITM genes. The promoter regions of both genes were examined and found to contain interferon, immune and stress-related transcription factor binding sites. Transcripts for both genes were found in most tissues examined. Trout IFITM1 mRNA levels were highest in head kidney, but present in all tissues studied. IFITM2 mRNA levels were highest in gill, heart and liver, and absent in head kidney and blood. In response to conditions mimicking viral infection, transcription of the trout IFITM genes was elevated in a trout spleen macrophage cell line (RTS11) over the examined timecourse (72 h). This study suggests that the organization and transcription of the IFITM genes is well conserved between human and rainbow trout. (c) 2006 Elsevier B.V. All rights reserved.en_IE
dc.description.sponsorshipThis work was supported by grants from the European Community (BIO2 CT 97-0554), BioResearch Ireland and Cork Co. Co.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofVeterinary Immunology And Immunopathologyen
dc.subjectOncorhynchus mykissen_IE
dc.subjectInterferon-inducibleen_IE
dc.subjectTransmembraneen_IE
dc.subjectPromotersen_IE
dc.subjectTeleosten_IE
dc.subjectSignal transductionen_IE
dc.subjectExpressionen_IE
dc.subjectAdhesionen_IE
dc.subjectCellsen_IE
dc.subjectBiomedical scienceen_IE
dc.titleCloning and characterization of two genes encoding rainbow trout homologues of the IFITM protein familyen_IE
dc.typeArticleen_IE
dc.date.updated2016-04-12T19:51:44Z
dc.identifier.doi10.1016/j.vetimm.2005.12.007
dc.local.publishedsourcehttp://www.sciencedirect.com/science/article/pii/S0165242706000092en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid1340486
dc.local.contactMichael Cairns, Glycoscience Group, Ncbes, Biomedical Sciences, Dangan, Newcastle Rd. 2094 Email: michael.cairns@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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