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dc.contributor.authorKelly, Helena
dc.contributor.authorDowning, Tim
dc.contributor.authorTuite, Nina
dc.contributor.authorSmith, Terry
dc.contributor.authorKerin, Michael
dc.contributor.authorDwyer, Róisín M.
dc.contributor.authorClancy, Eoin
dc.contributor.authorReddington, Kate
dc.contributor.authorBarry, Thomas
dc.date.accessioned2016-04-11T08:57:01Z
dc.date.available2016-04-11T08:57:01Z
dc.date.issued2015-09-10
dc.identifier.citationHelena Kelly, Tim Downing, Nina L Tuite, Terry J Smith, Michael J Kerin, Róisín M Dwyer, Eoin Clancy, Thomas Barry, Kate Reddington (2015) 'Cross Platform Standardisation of an Experimental Pipeline for Use in the Identification of Dysregulated Human Circulating MiRNAs'. Plos One, 10 (9).en_IE
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10379/5652
dc.description.abstractMicro RNAs (miRNAs) are a class of highly conserved small non-coding RNAs that play an important part in the post-transcriptional regulation of gene expression. A substantial number of miRNAs have been proposed as biomarkers for diseases. While reverse transcriptase Real-time PCR (RT-qPCR) is considered the gold standard for the evaluation and validation of miRNA biomarkers, small RNA sequencing is now routinely being adopted for the identification of dysregulated miRNAs. However, in many cases where putative miRNA biomarkers are identified using small RNA sequencing, they are not substantiated when RT-qPCR is used for validation. To date, there is a lack of consensus regarding optimal methodologies for miRNA detection, quantification and standardisation when different platform technologies are used.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherPublic Library of Scienceen_IE
dc.relation.ispartofPlos One.en
dc.subjectMicroRNAsen_IE
dc.subjectRNA sequencingen_IE
dc.subjectBreast canceren_IE
dc.subjectRNA isolationen_IE
dc.subjectBiomarkersen_IE
dc.subjectRNA foldingen_IE
dc.subjectBlooden_IE
dc.subjectRNA sequence analysisen_IE
dc.subjectMicrobiologyen_IE
dc.titleCross platform standardisation of an experimental pipeline for use in the identification of dysregulated human circulating MiRNAsen_IE
dc.typeArticleen_IE
dc.date.updated2016-04-06T13:28:33Z
dc.identifier.doi10.1371/journal.pone.0137389
dc.local.publishedsourcehttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0137389en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|1267880|~|
dc.internal.rssid9945911
dc.local.contactThomas Barry, Dept. Of Microbiology, Arts/Science Building, Nui Galway. 3189 Email: thomas.barry@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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