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dc.contributor.authorCairns, Michael T.
dc.contributor.authorDavey, Grace C.
dc.date.accessioned2016-02-16T15:38:10Z
dc.date.available2016-02-16T15:38:10Z
dc.date.issued2012
dc.identifier.citationCalduch-Giner, JA,Sitja-Bobadilla, A,Davey, GC,Cairns, MT,Kaushik, S,Perez-Sanchez, J (2012) 'Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (Sparus aurata), but modulate the transcriptomic response to infection with Enteromyxum leei'. BMC Genomics, 13 .en_IE
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/10379/5558
dc.description.abstractBackground: Studies conducted with gilthead sea bream (Sparus aurata L.) have determined the maximum dietary replacement of fish meal and oil without compromising growth or product quality. The present study aimed to analyze the effect of the nutritional background on fish health and fish fed plant protein-based diets with fish oil (FO diet) or a blend of vegetable oils (66VO diet) were exposed for 102 days to the intestinal myxosporean parasite Enteromyxum leei, and the intestine transcriptome was analyzed with a customized oligo-microarray of 7,500 annotated genes.Results: Infection prevalence was high and similar in the two diet groups, but the outcome of the disease was more pronounced in fish fed the 66VO diet. No differences were found in the transcriptome of both diet control groups, whereas the number of differentially expressed genes in infected groups was considerable. K-means clustering of these differentially expressed genes identified four expression patterns that reflected the progression of the disease with the magnitude of the fold-change being higher in infected 66VO fish. A positive correlation was found between the time of infection and the magnitude of the transcriptional change within the 66VO group, being higher in early infected animals. Within this diet group, a strong up-regulation of many components of the immune specific response was evidenced, whereas other genes related to complement response and xenobiotic metabolism were down-regulated.Conclusions: The high replacement of fish oil by vegetable oils in practical fish feeds did not modify the intestine transcriptome of gilthead sea bream, but important changes were apparent when fish were exposed to the myxosporean E. leei. The detected changes were mostly a consequence rather than a cause of the different disease progression in the two diet groups. Hence, the developed microarray constitutes an excellent diagnostic tool to address changes associated with the action of intestinal pathogens, but lacks a prognostic value to predict in advance the different susceptibility of growing fish to the current pathogen.en_IE
dc.description.sponsorshipThis work was funded by EU through projects AQUAMAX (FOOD-CT-2006-16249; Sustainable Aquafeeds to Maximise the Health Benefits of Farmed Fish for Consumers) and ARRAINA (Advanced Research Initiatives for Nutrition & Aquaculture, FP7/2007/2013; grant agreement n° 288925), and by the Spanish Ministry of Science and Innovation (MICINN) through the projects AGL2009-13282-C02-01 and AQUAGENOMICS (CSD2007-00002, Improvement of Aquaculture Production by the Use of Biotechnological Tools). Additional funding was obtained from the “Generalitat Valenciana” (research grant PROMETEO 2010/006).en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherBioMed Centralen_IE
dc.relation.ispartofBMC Genomicsen
dc.subjectTeleosten_IE
dc.subjectParasiteen_IE
dc.subjectMyxozoaen_IE
dc.subjectIntestineen_IE
dc.subjectTranscriptomeen_IE
dc.subjectNutrigenomicsen_IE
dc.subjectSalmon salmo-salaren_IE
dc.subjectTrout oncorhynchus-mykissen_IE
dc.subjectOrange-spotted grouperen_IE
dc.subjectFatty-acid-compositionen_IE
dc.subjectImmune relevant genesen_IE
dc.subjectCyprinus-carpio Len_IE
dc.subjectAtlantic salmonen_IE
dc.subjectRainbow trouten_IE
dc.subjectMicroarray technologyen_IE
dc.subjectFish oilen_IE
dc.titleDietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (Sparus aurata), but modulate the transcriptomic response to infection with Enteromyxum leeien_IE
dc.typeArticleen_IE
dc.date.updated2016-02-02T15:58:13Z
dc.identifier.doi10.1186/1471-2164-13-470
dc.local.publishedsourcehttp://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-470en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid2598363
dc.local.contactMichael Cairns, Mri, Nui Galway. 2094 Email: michael.cairns@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
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