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dc.contributor.authorKazezian, Zepur
dc.contributor.authorGawri, Rahul
dc.contributor.authorHaglund, Lisbet
dc.contributor.authorOuellet, Jean
dc.contributor.authorMwale, Fackson
dc.contributor.authorTarrant, Finbarr
dc.contributor.authorO’Gaora, Peadar
dc.contributor.authorPandit, Abhay
dc.contributor.authorAlini, Mauro
dc.contributor.authorGrad, Sibylle
dc.identifier.citationKazezian, Z,Gawri, R,Haglund, L,Ouellet, J,Mwale, F,Tarrant, F,O'Gaora, P,Pandit, A,Alini, M,Grad, S (2015) 'Gene Expression Profiling Identifies Interferon Signalling Molecules and IGFBP3 in Human Degenerative Annulus Fibrosus'. Scientific Reports, 5 .en_IE
dc.description.abstractLow back pain is a major cause of disability especially for people between 20 and 50 years of age. As a costly healthcare problem, it imposes a serious socio-economic burden. Current surgical therapies fail to replace the normal disc in facilitating spinal movements and absorbing load. The focus of regenerative medicine is on identifying biomarkers and signalling pathways to improve our understanding about cascades of disc degeneration and allow for the design of specific therapies. We hypothesized that comparing microarray profiles from degenerative and non-degenerative discs will lead to the identification of dysregulated signalling and pathophysiological targets. Microarray data sets were generated from human annulus fibrosus cells and analysed using IPA ingenuity pathway analysis. Gene expression values were validated by qRT-PCR, and respective proteins were identified by immunohistochemistry. Microarray analysis revealed 238 differentially expressed genes in the degenerative annulus fibrosus. Seventeen of the dysregulated molecular markers showed log(2)-fold changes greater than +/- 1.5. Various dysregulated cellular functions, including cell proliferation and inflammatory response, were identified. The most significant canonical pathway induced in degenerative annulus fibrosus was found to be the interferon pathway. This study indicates interferon-alpha signalling pathway activation with IFIT3 and IGFBP3 up-regulation, which may affect cellular function in human degenerative disc.en_IE
dc.description.sponsorshipThis study was funded by the AO Foundation Collaborative Research Program Annulus Fibrosus Repairen_IE
dc.publisherNature Publishing Group:en_IE
dc.relation.ispartofScientific Reportsen
dc.subjectLower back painen_IE
dc.subjectLung canceren_IE
dc.subjectCartilage cellsen_IE
dc.subjectCytokine expressionen_IE
dc.subjectCaveolin-1 expressionen_IE
dc.subjectHuman nucleus pulposusen_IE
dc.subjectFactor binding protein-3en_IE
dc.titleGene expression profiling identifies interferon signalling molecules and IGFBP3 in human degenerative annulus fibrosusen_IE
dc.local.contactAbhay Shashikant Pandit, Curam, Biosciences, Nui Galway. 2758 Email:

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