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dc.contributor.authorGroza, Radu Constantin
dc.contributor.authorRyder, Alan G.
dc.contributor.authorLi, Boyan
dc.date.accessioned2016-01-29T21:19:37Z
dc.date.issued2015-07-30
dc.identifier.citationGroza, RC; Li, BY; Ryder, AG; (2015) 'Anisotropy resolved multidimensional emission spectroscopy (ARMES): A new tool for protein analysis'. Analytica chimica acta, 886 :133-142.en_IE
dc.identifier.issn1873-4324
dc.identifier.urihttp://hdl.handle.net/10379/5507
dc.description.abstractStructural analysis of proteins using the emission of intrinsic fluorophores is complicated by spectral overlap. Anisotropy resolved multidimensional emission spectroscopy (ARMES) overcame the overlap problem by the use of anisotropy, with chemometric analysis, to better resolve emission from different fluorophores. Total synchronous fluorescence scan (TSFS) provided information about all the fluorophores that contributed to emission while anisotropy provided information about the environment of each fluorophore. Here the utility of ARMES was demonstrated via study of the chemical and thermal denaturation of human serum albumin (HSA).Multivariate curve resolution (MCR) analysis of the constituent polarized emission ARMES data resolved contributions from four emitters: fluorescence from tryptophan (Trp), solvent exposed tyrosine (Tyr), Tyr in a hydrophobic environment, and room temperature phosphorescence (RTP) from Trp. The MCR scores, anisotropy, and literature validated these assignments and showed all the expected transitions during HSA unfolding. This new methodology for comprehensive intrinsic fluorescence analysis of proteins is applicable to any protein containing multiple fluorophores. (C) 2015 Elsevier B.V. All rights reserved.en_IE
dc.description.sponsorshipRCG was supported by an ‘EMBARK Initiative’ Postgraduate Scholarship from the Irish Research Councilen_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofAnalytica chimica actaen
dc.subjectFluorescenceen_IE
dc.subjectAnisotropyen_IE
dc.subjectMultidimensionalen_IE
dc.subjectProteinen_IE
dc.subjectUnfoldingen_IE
dc.subjectChemometricsen_IE
dc.subjectHuman-serum-albuminen_IE
dc.subjectCell culture mediaen_IE
dc.subjectMultivariate curve resolutionen_IE
dc.subjectPrincipal component analysisen_IE
dc.subjectFluorescence spectroscopyen_IE
dc.subjectNoise perturbationen_IE
dc.subjectCrystal-structureen_IE
dc.subjectPractical aspectsen_IE
dc.subjectEEM spectroscopyen_IE
dc.subjectLow temperaturesen_IE
dc.subjectChemistryen_IE
dc.titleAnisotropy resolved multidimensional emission spectroscopy (ARMES): A new tool for protein analysisen_IE
dc.typeArticleen_IE
dc.date.updated2015-12-22T16:39:55Z
dc.identifier.doi10.1016/j.aca.2015.06.011
dc.local.publishedsourcehttp://dx.doi.org/10.1016/j.aca.2015.06.011en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|6201984|~|
dc.description.embargo2017-07-30
dc.internal.rssid9878581
dc.local.contactAlan Ryder, School Of Chemistry, Room 213, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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