Browsing Biochemistry by Title
Now showing items 21-40 of 41
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Methods for Monitoring Endoplasmic Reticulum Stress and the Unfolded Protein Response
(2010)The endoplasmic reticulum (ER) is the site of folding of membrane and secreted proteins in the cell. Physiological or pathological processes that disturb protein folding in the endoplasmic reticulum cause ER stress and ... -
Mrc1, Tof1 and Csm3 inhibit CAG·CTG repeat instability by at least two mechanisms
(Springer, 2008)Trinucleotide repeats frequently expand and contract in humans and model organisms. Protein factors that modulate this process have been found by candidate gene approaches or mutant screens for increased expansion ... -
Nerve growth factor (NGF)-mediated regulation of p75(NTR) expression contributes to chemotherapeutic resistance in triple negative breast cancer cells
(Elsevier, 2016)Triple negative breast cancer [TNBC] cells are reported to secrete the neurotrophin nerve growth factor [NGF] and express its receptors, p75 neurotrophin receptor [p75(NTR)] and TrkA, leading to NGF-activated pro-survival ... -
Nerve Growth Factor in Cancer Cell Death and Survival
(MDPI, 2011-02)One of the major challenges for cancer therapeutics is the resistance of many tumor cells to induction of cell death due to pro-survival signaling in the cancer cells. Here we review the growing literature which shows that ... -
Nerve growth factor-mediated inhibition of apoptosis post-caspase activation is due to removal of active caspase-3 in a lysosome-dependent manner
(Nature Publishing Group, 2014-05-01)Nerve growth factor (NGF) is well characterised as an important pro-survival factor in neuronal cells that can inhibit apoptotic cell death upstream of mitochondrial outer membrane permeabilisation. Here we addressed the ... -
Neuronal cell death in neurodegenerative diseases: recurring themes around protein handling
(Wiley, 2008-06-27)Neuronal cell death plays a role in many chronic neurodegenerative diseases with the loss of particular subsets of neurons. The loss of the neurons occurs during a period of many years, which can make the mode(s) of cell ... -
Neurotrophins and B-cell malignancies
(Springer Verlag, 2015-09-23)Neurotrophins and their receptors act as important proliferative and pro-survival factors in a variety of cell types. Neurotrophins are produced by multiple cell types in both pro and mature forms, and can act in an autocrine ... -
Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences
(Wiley, 2018-04-16)The modulation of expression levels of fluorescent fusion proteins (FFPs) is central for recombinant DNA technologies in modern biology as overexpression of proteins contributes to artifacts in biological experiments. In ... -
Partial reconstitution of DNA large loop repair with purified proteins from Saccharomyces cerevisiae
(OUP, 2008)Small looped mispairs are corrected by DNA mismatch repair. In addition, a distinct process called large loop repair (LLR) corrects heteroduplexes up to several hundred nucleotides in bacteria, yeast and human cells, and ... -
Population‐based identity‐by‐descent mapping combined with exome sequencing to detect rare risk variants for schizophrenia
(Wiley, 2019-02-23)Genome‐wide association studies (GWASs) are highly effective at identifying common risk variants for schizophrenia. Rare risk variants are also important contributors to schizophrenia etiology but, with the exception of ... -
Premitotic Assembly of Human CENPs -T and -W Switches Centromeric Chromatin to a Mitotic State.
(2011)Centromeres are differentiated chromatin domains, present once per chromosome, that direct segregation of the genome in mitosis and meiosis by specifying assembly of the kinetochore. They are distinct genetic loci in that ... -
The Proteasome Inhibitor Bortezomib Sensitizes AML with Myelomonocytic Differentiation to TRAIL Mediated Apoptosis
(2011)Acute myeloid leukemia (AML) is an aggressive stem cell malignancy that is difficult to treat. There are limitations to the current treatment regimes especially after disease relapse, and therefore new therapeutic agents ... -
Rapid and efficient cancer cell killing mediated by high-affinity death receptor homotrimerizing TRAIL variants
(Macmillan Publishers Limited, 2010)The tumour necrosis factor family member TNF-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in a variety of cancer cells through the activation of death receptors 4 (DR4) and 5 (DR5) and is considered ... -
Rapid unwinding of triplet repeat hairpins by Srs2 helicase of Saccharomyces cerevisiae
(2008)Expansions of trinucleotide repeats cause at least 15 heritable human diseases. Single-stranded triplet repeat DNA in vitro forms stable hairpins in a sequence-dependent manner that correlates with expansion risk in vivo. ... -
Restriction of human polyomavirus BK virus DNA replication in murine cells and extracts
(2009)BK virus (BKV) causes persistent and asymptomatic infections in most humans and is the etiologic agent of polyomavirus-associated nephropathy (PVAN) and other pathologies. Unfortunately, there are no animal models with ... -
RIP2 enhances cell survival by activation of NF-ĸB in triple negative breast cancer cells
(Elsevier, 2018-02-06)Receptor-interacting protein 2 (RIP2) is an essential mediator of inflammation and innate immunity, but little is known about its role outside the immune system. Recently, RIP2 has been linked to chemoresistance of triple ... -
RNA from a simple-tandem repeat is required for sperm maturation and male fertility in Drosophila melanogaster
(eLife Sciences Publications, 2019-11-05)Tandemly-repeated DNAs, or satellites, are enriched in heterochromatic regions of eukaryotic genomes and contribute to nuclear structure and function. Some satellites are transcribed, but we lack direct evidence that ... -
Role of mitochondria in neuronal apoptosis
(Karger Publishers, 2000-12)Apoptosis is a controlled form of cell death that participates in the demise of neuronal cells during development, neurodegenerative disorders and exposure to neurotoxic agents. In recent years, the mitochondria have emerged ... -
Stress management at the ER: regulators of ER stress-induced apoptosis.
(Elsevier, 2012-02-17)The endoplasmic reticulum (ER) is an elaborate cellular organelle essential for cell function and survival. Conditions that interfere with ER function lead to the accumulation and aggregation of unfolded proteins which are ... -
SV40 T antigen helicase domain regions responsible for oligomerisation regulate Okazaki fragment synthesis initiation
(Wiley Open Access and Federation of European Biochemical Societies, 2022-01-24)The initiation of Okazaki fragment synthesis during cellular DNA replication is a crucial step for lagging strand synthesis, which is carried out by the primase function of DNA polymerase ¿-primase (Pol-prim). Since cellular ...