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dc.contributor.authorO'Flynn, Lisa
dc.contributor.authorTreacy, Oliver
dc.contributor.authorRyan, Aideen E.
dc.contributor.authorMorcos, Maurice
dc.contributor.authorCregg, Marese
dc.contributor.authorGerlach, Jared
dc.contributor.authorJoshi, Lokesh
dc.contributor.authorNosov, Mikhail
dc.contributor.authorRitter, Thomas
dc.identifier.citationO'Flynn, Lisa, Treacy, Oliver, Ryan, Aideen E., Morcos, Maurice, Cregg, Marese, Gerlach, Jared, . . . Ritter, Thomas. (2013). Donor Bone Marrow–derived Dendritic Cells Prolong Corneal Allograft Survival and Promote an Intragraft Immunoregulatory Milieu. Molecular Therapy, 21(11), 2102-2112. doi:
dc.descriptionJournal articleen_IE
dc.description.abstractInvestigations into cell therapies for application in organ transplantation have grown. Here, we describe the ex vivo generation of donor bone marrow-derived dendritic cells (BMDCs) and glucocorticoid-treated BMDCs with potent immunomodulatory properties for application in allogeneic transplantation. BMDCs were treated with dexamethasone (Dexa) to induce an immature, maturation-resistant phenotype. BMDC and Dexa BMDC phenotype, antigen presenting cell function, and immunomodulatory properties were fully characterized. Both populations display significant immunomodulatory properties, including, but not limited to, a significant increase in mRNA expression of programmed death-ligand 1 and indoleamine 2,3-dioxygenase. BMDCs and Dexa BMDCs display a profound impaired capacity to stimulate allogeneic lymphocytes. Moreover, in a fully MHC I/II mismatched rat corneal transplantation model, injection of donor-derived, untreated BMDC or Dexa BMDCs (1â Ã â 10(6) cells, day -7) significantly prolonged corneal allograft survival without the need for additional immunosuppression. Although neovascularization was not reduced and evidence of donor-specific alloantibody response was detected, a significant reduction in allograft cellular infiltration combined with a significant increase in the ratio of intragraft FoxP3-expressing regulatory cells was observed. Our comprehensive analysis demonstrates the novel cellular therapeutic approach and significant effect of donor-derived, untreated BMDCs and Dexa BMDCs in preventing corneal allograft rejection.en_IE
dc.description.sponsorshipScience Foundation Ireland - Grant No. [07/IN.1/B925], 09/SRC-B1794; European Regional Development Funden_IE
dc.publisherNature Publishing Groupen_IE
dc.relation.ispartofMolecular Therapyen
dc.subjectOrgan transplantationen_IE
dc.subjectDonor bone marrow-derived dendritic cells (BMDCs)en_IE
dc.subjectGlucocorticoid-treated BMDCsen_IE
dc.titleDonor bone marrow-derived dendritic cells prolong corneal allograft survival and promote an intragraft immunoregulatory milieu.en_IE
dc.local.contactThomas Ritter, School Of Medicine, Regenerative Medicine Institute, Biosciences, Dangan. 5329 Email:

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