Show simple item record

dc.contributor.authorClancy, Eoin
dc.contributor.authorCoughlan, Helena
dc.contributor.authorHelena, Thomas
dc.contributor.authorSmith, Terry
dc.contributor.authorDwyer, Róisín M.
dc.date.accessioned2014-10-22T12:35:21Z
dc.date.available2014-10-22T12:35:21Z
dc.date.issued2014-09-12
dc.identifier.citationDimov, N., Clancy, E., Gaughran, J., Boyle, D., Mc Auley, D., Glynn, M., et al. Solvent-selective routing for centrifugally automated solid-phase purification of RNA. Microfluidics and Nanofluidics, 1-13.en_US
dc.identifier.issn1613-4982
dc.identifier.urihttp://hdl.handle.net/10379/4668
dc.descriptionJournal articleen_US
dc.description.abstractWe present a disc-based module for rotationally controlled solid-phase purification of RNA from cell lysate. To this end, multi-stage routing of a sequence of aqueous and organic liquids into designated waste and elution reservoirs is implemented by a network of strategically placed, solvent-selective composite valves . Using a bead-based stationary phase at the entrance of the router, we show that total RNA is purified with high integrity from cultured MCF7 and T47D cell lines, human leucocytes and Haemophilus influenzae cell lysates. Furthermore, we demonstrate the broad applicability of the device through the in vitro amplification of RNA purified on-disc using RT-PCR and NASBA. Our novel router will be at the pivot of a forthcoming, fully integrated and automated sample preparation system for RNA-based analysis en_US
dc.description.sponsorshipScience Foundation Ireland - Grant 10/CE/B1821en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMicrofluidics And Nanofluidicsen
dc.subjectLab-on-a-discen_US
dc.subjectRNA purificationen_US
dc.subjectSolvent-selective valvesen_US
dc.subjectMolecular diagnosticsen_US
dc.titleSolvent-selective routing for centrifugally automated solid-phase purification of RNA.en_US
dc.typeArticleen_US
dc.date.updated2014-10-21T17:07:09Z
dc.local.publishedsourcehttp://dx.doi.org/10.1007/s10404-014-1477-9en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|SFI|~|
dc.internal.rssid7467701
dc.local.contactTerry Smith, School Of Natural Sciences, Nui Galway. 2022 Email: terry.smith@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
nui.item.downloads1382


Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:

Thumbnail

This item appears in the following Collection(s)

Show simple item record