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dc.contributor.authorFriel, Anne M,
dc.contributor.authorSexton, Donal J.
dc.contributor.authorO'Reilly, Michael W.
dc.contributor.authorSmith, Terry
dc.contributor.authorMorrison, John J.
dc.date.accessioned2014-10-22T10:59:54Z
dc.date.available2014-10-22T10:59:54Z
dc.date.issued2006-07-01
dc.identifier.citationFriel, A. M., Sexton, D. J., O'Reilly, M. W., Smith, T. J., & Morrison, J. J. (2006). Rho A/Rho kinase: human umbilical artery mRNA expression in normal and pre eclamptic pregnancies and functional role in isoprostane-induced vasoconstriction. Reproduction, 132(1), 169-176.en_US
dc.identifier.issn1741-7899
dc.identifier.urihttp://hdl.handle.net/10379/4664
dc.descriptionJournal articleen_US
dc.description.abstractPre eclampsia represents a state of increased or prolonged vasoconstriction, partially linked to the potent vasocontractile effect of isoprostanes. The process of Rho A-mediated calcium sensitisation is inherent to a state of prolonged contractility in many smooth muscle types. The aim of this study was (1) to investigate mRNA expression levels of Rho A and Rho kinase isoforms (I and 11) in the umbilical artery from normotensive and pre eclamptic women and (2) to determine whether the effects of two isoprostanes, 8-iso prostaglandin F-2 alpha (8-iso PGF2 alpha) and 8-iso, prostaglandin E-2 (8-iso PGE(2)), on umbilical artery tone, were mediated via the Rho kinase pathway. Real-time RT-PCR using primers for Rho A, ROCK I and ROCK 11 was performed on total RNA isolated from umbilical artery specimens obtained from normotensive and pre eclamptic women. The effects of both isoprostanes (n=6) (in the absence and presence of the specific Rho kinase inhibitor Y-27632), on umbilical artery tone were measured, and compared with control recordings. Rho A mRNA expression levels were significantly lower in umbilical artery samples obtained from pre eclamptic women (n=4) in comparison to those from normotensive women (n=6) (P 0.05). Both isoprostanes exerted a significant concentration-dependent vasocontractile effect (n=7) (Pen_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherSociety for Reproduction and Fertilityen_US
dc.relation.ispartofReproductionen
dc.subjectSmooth muscleen_US
dc.subject8-ISO-Prostaglandin F2-ALPHAen_US
dc.subjectOxidant stressen_US
dc.subjectHeart failureen_US
dc.subjectIn vitroen_US
dc.subjectPre-eclampsiaen_US
dc.subjectHypertensionen_US
dc.subjectMyometriumen_US
dc.subjectProteinsen_US
dc.subjectVivoen_US
dc.titleRho A/Rho kinase: human umbilical artery mRNA expression in normal and pre eclamptic pregnancies and functional role in isoprostane-induced vasoconstrictionen_US
dc.typeArticleen_US
dc.date.updated2014-10-21T16:01:00Z
dc.identifier.doiDOI 10.1530/rep.1.01088
dc.local.publishedsourcehttp://dx.doi.org/10.1530/rep.1.01088en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid1333413
dc.local.contactTerry Smith, School Of Natural Sciences, Nui Galway. 2022 Email: terry.smith@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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