dc.contributor.author | Prasad, Babu R. | |
dc.contributor.author | Mullins, Gillian | |
dc.contributor.author | Nikolskaya, Natalia | |
dc.contributor.author | Connolly, David | |
dc.contributor.author | Smith, Terry | |
dc.contributor.author | Rochev, Yury | |
dc.date.accessioned | 2014-10-22T10:50:24Z | |
dc.date.available | 2014-10-22T10:50:24Z | |
dc.date.issued | 2012-01-20 | |
dc.identifier.citation | Prasad, BR,Mullins, G,Nikolskaya, N,Connolly, D,Smith, TJ,Gerard, VA,Byrne, SJ,Davies, GL,Gun'ko, YK,Rochev, Y (2012) 'Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells'. Journal Of Nanobiotechnology, 10 (4). | en_US |
dc.identifier.issn | 1477-3155 | |
dc.identifier.uri | http://hdl.handle.net/10379/4663 | |
dc.description | Journal article (open access) | en_US |
dc.description.abstract | Background: The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy.Results: Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs.Conclusion: The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days). | en_US |
dc.description.sponsorship | Science Foundation Ireland | en_US |
dc.format | application/pdf | en_US |
dc.language.iso | en | en_US |
dc.publisher | BioMed Central | en_US |
dc.relation.ispartof | Journal Of Nanobiotechnology | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | CdTe Quantum Dots | en_US |
dc.subject | Differentiated PC12 cells | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | Neuronal Growth Factor | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Cytoplasmic calcium levels | en_US |
dc.subject | Oxidative stress | en_US |
dc.subject | Intracellular transport | en_US |
dc.subject | Hippocampal neurons | en_US |
dc.subject | Cytochrome-CCDTE | en_US |
dc.subject | Surface | en_US |
dc.subject | CDSE | en_US |
dc.subject | Nanocrystals | en_US |
dc.subject | Cytotoxicity | en_US |
dc.title | Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells | en_US |
dc.type | Article | en_US |
dc.date.updated | 2014-10-21T15:54:05Z | |
dc.identifier.doi | 10.1186/1477-3155-10-4 | |
dc.local.publishedsource | http://dx.doi.org/10.1186/1477-3155-10-4 | en_US |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | |~| | |
dc.internal.rssid | 2196212 | |
dc.local.contact | Terry Smith, School Of Natural Sciences, Nui Galway. 2022 Email: terry.smith@nuigalway.ie | |
dc.local.copyrightchecked | No | |
dc.local.version | PUBLISHED | |
nui.item.downloads | 269 | |