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dc.contributor.authorPrasad, Babu R.
dc.contributor.authorMullins, Gillian
dc.contributor.authorNikolskaya, Natalia
dc.contributor.authorConnolly, David
dc.contributor.authorSmith, Terry
dc.contributor.authorRochev, Yury
dc.date.accessioned2014-10-22T10:50:24Z
dc.date.available2014-10-22T10:50:24Z
dc.date.issued2012-01-20
dc.identifier.citationPrasad, BR,Mullins, G,Nikolskaya, N,Connolly, D,Smith, TJ,Gerard, VA,Byrne, SJ,Davies, GL,Gun'ko, YK,Rochev, Y (2012) 'Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells'. Journal Of Nanobiotechnology, 10 (4).en_US
dc.identifier.issn1477-3155
dc.identifier.urihttp://hdl.handle.net/10379/4663
dc.descriptionJournal article (open access)en_US
dc.description.abstractBackground: The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy.Results: Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs.Conclusion: The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).en_US
dc.description.sponsorshipScience Foundation Irelanden_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.relation.ispartofJournal Of Nanobiotechnologyen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectCdTe Quantum Dotsen_US
dc.subjectDifferentiated PC12 cellsen_US
dc.subjectCytotoxicityen_US
dc.subjectNeuronal Growth Factoren_US
dc.subjectApoptosisen_US
dc.subjectCytoplasmic calcium levelsen_US
dc.subjectOxidative stressen_US
dc.subjectIntracellular transporten_US
dc.subjectHippocampal neuronsen_US
dc.subjectCytochrome-CCDTEen_US
dc.subjectSurfaceen_US
dc.subjectCDSEen_US
dc.subjectNanocrystalsen_US
dc.subjectCytotoxicityen_US
dc.titleEffects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cellsen_US
dc.typeArticleen_US
dc.date.updated2014-10-21T15:54:05Z
dc.identifier.doi10.1186/1477-3155-10-4
dc.local.publishedsourcehttp://dx.doi.org/10.1186/1477-3155-10-4en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid2196212
dc.local.contactTerry Smith, School Of Natural Sciences, Nui Galway. 2022 Email: terry.smith@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionPUBLISHED
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland