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dc.contributor.authorLeyden, Rosaria
dc.contributor.authorMurphy, Paul V.
dc.date.accessioned2014-05-19T10:07:47Z
dc.date.available2014-05-19T10:07:47Z
dc.date.issued2012-08-24
dc.identifier.citationWright, C., Leyden, R., Murphy, P. V., Callaghan, M., Velasco-Torrijos, T., & McClean, S. Inhibition of Burkholderia multivorans Adhesion to Lung Epithelial Cells by Bivalent Lactosides. Molecules, 17(9), 10065-10071.en_US
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10379/4362
dc.descriptionJournal article (open access)en_US
dc.description.abstractBurkholderia cepacia complex (Bcc) is an opportunistic pathogen in cystic fibrosis patients which is inherently resistant to antimicrobial agents. The mechanisms of attachment and pathogenesis of Bcc, a group of 17 species, are poorly understood. The most commonly identified Bcc species in newly colonised patients, Burkholderia multivorans, continues to be acquired from the environment. Development of therapies which can prevent or reduce the risk of colonization on exposure to Bcc in the environment would be a better alternative to antimicrobial agents. Previously, it has been shown that Bcc strains bound to many glycolipid receptors on lung epithelia. Using a real-time PCR method to quantify the levels of binding of B. multivorans to the lung epithelial cells, we have examined glycoconjugate derivatives for their potential to inhibit host cell attachment. Bivalent lactosides previously shown to inhibit galectin binding significantly reduced the attachment of B. multivorans to CF lung epithelial cells at micromolar concentrations. This was in contrast to monosaccharides and lactose, which were only effective in the millimolar range. Development of glycoconjugate therapies such as these, which inhibit attachment to lung epithelial cells, represent an alternative means of preventing infection with inherently antimicrobially resistant pathogens such as B. multivorans.en_US
dc.description.sponsorshipProgramme for Research in Third Level Institutions (PRTLI) Cycle 4en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofMolecules (Basel, Switzerland)en
dc.subjectBacterial colonizationen_US
dc.subjectBivalent lactosideen_US
dc.subjectBurkholderia cepacia complexen_US
dc.subjectCystic fibrosisen_US
dc.subjectGlycoconjugateen_US
dc.subjectQuantificationen_US
dc.subjectPCRen_US
dc.titleInhibition of Burkholderia multivorans Adhesion to Lung Epithelial Cells by Bivalent Lactosidesen_US
dc.typeArticleen_US
dc.date.updated2014-04-04T11:49:37Z
dc.identifier.doiDOI 10.3390/molecules170910065
dc.local.publishedsourcehttp://dx.doi.org/10.3390/molecules170910065en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid2680250
dc.local.contactPaul Murphy, School Of Chemistry, Room 236, Arts/Science Building, Nui Galway. 2465 Email: paul.v.murphy@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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