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dc.contributor.authorHolladay, Carolyn
dc.contributor.authorAbbah, Sunny-Akogwu
dc.date.accessioned2014-03-14T14:41:52Z
dc.date.available2014-03-14T14:41:52Z
dc.date.issued2014
dc.identifier.citationHolladay C, Abbah SA, O'Dowd C, Pandit A, Zeugolis DI. (2014) 'Preferential tendon stem cell response to growth factor supplementation'. Journal Of Tissue Engineering And Regenerative Medicine, .en_US
dc.identifier.issn1932-7005
dc.identifier.urihttp://hdl.handle.net/10379/4242
dc.descriptionJournal articleen_US
dc.description.abstractTendon injuries are increasingly prevalent around the world, accounting for more than 100 000 new clinical cases/year in the USA alone. Cell-based therapies have been proposed as a therapeutic strategy, with recent data advocating the use of tendon stem cells (TSCs) as a potential cell source with clinical relevance for tendon regeneration. However, their in vitro expansion is problematic, as they lose their multipotency and change their protein expression profile in culture. Herein, we ventured to assess the influence of insulin-like growth factor 1 (IGF-1), growth and differentiation factor-5 (GDF-5) and transforming growth factor-β1 (TGFβ1) supplementation in TSC culture. IGF-1 preserved multipotency for up to 28 days. Upregulation of decorin and scleraxis expression was observed as compared to freshly isolated cells. GDF-5 treated cells exhibited reduced differentiation along adipogenic and chondrogenic pathways after 28 days, and decorin, scleraxis and collagen type I expression was increased. After 28 days, TGFβ1 supplementation led to increased scleraxis, osteonectin and collagen type II expression. The varied responses to each growth factor may reflect their role in tendon repair, suggesting that: GDF-5 promotes the transition of tendon stem cells towards tenocytes; TGFβ1 induces differentiation along several pathways, including a phenotype indicative of fibrocartilage or calcified tendon, common problems in tendon healing; and IGF-1 promotes proliferation and maintenance of TSC phenotypes, thereby creating a population sufficient to have a beneficial effecten_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Tissue Engineering And Regenerative Medicineen
dc.subjectTendon stem cell phenotype maintenanceen_US
dc.subjectTendon stem cell differentiationen_US
dc.subjectGrowth factor supplementationen_US
dc.subjectTendon stem cell cultureen_US
dc.subjectTenogenesisen_US
dc.subjectTendon healingen_US
dc.titlePreferential tendon stem cell response to growth factor supplementation.en_US
dc.typeArticleen_US
dc.date.updated2014-03-05T10:52:05Z
dc.local.publishedsourcehttp://dx.doi.org/10.1002/term.1852en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid5950625
dc.local.contactSunny Akogwu Abbah, Nfb, College Of Engineering &, Informatics, Nui Galway. Email: sunnyakogwu.abbah@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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