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dc.contributor.authorMcMahon, Siobhan
dc.contributor.authorNikolskaya, Natalia
dc.contributor.authorNí Choileain, Siobhan
dc.contributor.authorHennessy, Niamh
dc.contributor.authorO'Brien, Timothy
dc.contributor.authorRochev, Yury
dc.date.accessioned2014-02-26T15:49:12Z
dc.date.available2014-02-26T15:49:12Z
dc.date.issued2011-11-01
dc.identifier.citationMcMahon, SS,Nikolskaya, N,Choileain, SN,Hennessy, N,O'Brien, T,Strappe, PM,Gorelov, A,Rochev, Y (2011) 'Thermosensitive hydrogel for prolonged delivery of lentiviral vector expressing neurotrophin-3 in vitro'. Journal Of Gene Medicine, 13 :591-601.en_US
dc.identifier.issn1099-498X
dc.identifier.urihttp://hdl.handle.net/10379/4229
dc.descriptionConference paperen_US
dc.description.abstractBackground The development of tissue engineering scaffolds for gene delivery has the potential to enhance gene transfer efficiency and safety via controlled temporal and spatial delivery. Lentiviral delivery can be carried out using the natural biopolymer thermoresponsive gel, chitosan/b-glycerol phosphate (b-GP) as a carrier.Methods Three chitosan/b-GP scaffolds were prepared with varying concentrations of chitosan and b-GP to obtain a pH and gelation temperature suitable for in situ delivery. A lentiviral vector expressing either green fluorescent protein (Lenti GFP) or neurotrophin-3 (Lenti NT-3) was incorporated into the chitosan/ b-GP scaffolds and also into collagen 0.1% w/v (control). Viral elution medium was removed at various timepoints and added to the culture medium of preseeded HeLa or primary dorsal root ganglia (DRG) cells, respectively. GFP gene expression was quantified using fluorescence-activated cell sorting analysis. The effect of Lenti NT-3 was analyzed by measuring DRG neurite outgrowth.Results Collagen displayed its most significant elution of virus on day 1 and chitosan/b-GP (with a final concentration of 2.17% chitosan) on day 3.Conclusions The system shows promise for the in situ, thermoresponsive delivery of lentiviral vectors providing long-term gene expression for therapeutic factors to treat conditions such as injury to the nervous system. Copyright (C) 2011 John Wiley & Sons, Ltd.en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Gene Medicineen
dc.subjectChitosanen_US
dc.subjectDorsal root gangliaen_US
dc.subjectGene therapyen_US
dc.subjectLentiviral vectoren_US
dc.subjectNeurotrophin-3en_US
dc.subjectSpinal cord injuryen_US
dc.subjectCentral nervous systemen_US
dc.subjectMultiple channel bridgesen_US
dc.subjectChitosan based hydrogelsen_US
dc.subjectGene deliveryen_US
dc.subjectTransgene expressionen_US
dc.subjectNeurite outgrowthen_US
dc.subjectStem cellsen_US
dc.subjectNanoparticlesen_US
dc.subjectEfficienten_US
dc.titleThermosensitive hydrogel for prolonged delivery of lentiviral vector expressing neurotrophin-3 in vitroen_US
dc.typeArticleen_US
dc.date.updated2014-02-24T22:24:45Z
dc.identifier.doiDOI 10.1002/jgm.1613
dc.local.publishedsourcehttp://dx.doi.org/10.1002/jgm.1613en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid1333047
dc.local.contactSiobhan Mcmahon, Department Of Anatomy, Nui, Galway. 2838 Email: siobhan.mcmahon@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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