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dc.contributor.authorRooney, Gemma E.
dc.contributor.authorMcMahon, Siobhan
dc.contributor.authorRitter, Thomas
dc.contributor.authorGarcia, Yolanda
dc.contributor.authorMoran, Cathal
dc.contributor.authorMadigan, Nicholas N.
dc.date.accessioned2014-02-26T15:09:32Z
dc.date.available2014-02-26T15:09:32Z
dc.date.issued2009-10
dc.identifier.citationRooney, GE,McMahon, SS,Ritter, T,Garcia, Y,Moran, C,Madigan, NN,Flugel, A,Dockery, P,O'Brien, T,Howard, L,Windebank, AJ,Barry, FP (2009) 'Neurotrophic Factor-Expressing Mesenchymal Stem Cells Survive Transplantation into the Contused Spinal Cord Without Differentiating into Neural Cells'. Tissue Engineering Part A, 15 :3049-3059.en_US
dc.identifier.urihttp://hdl.handle.net/10379/4226
dc.descriptionJournal articleen_US
dc.description.abstractThe aim of this study was to assess the feasibility of transplanting mesenchymal stem cells (MSCs), genetically modified to express glial-derived neurotrophic factor (GDNF), to the contused rat spinal cord, and to subsequently assess their neural differentiation potential. MSCs expressing green fluorescent protein were transduced with a retroviral vector to express the neurotrophin GDNF. The transduction protocol was optimized by using green fluorescent protein-expressing retroviral constructs; approximately 90% of MSCs were transduced successfully after G418 selection. GDNF-transduced MSCs expressed the transgene and secreted growth factor into the media (similar to 12 ng/500,000 cells secreted into the supernatant 2 weeks after transduction). Injuries were established using an impactor device, which applied a given, reproducible force to the exposed spinal cord. GDNF-expressing MSCs were transplanted rostral and caudal to the site of injury. Spinal cord sections were analyzed 2 and 6 weeks after transplantation. We demonstrate that GDNF-transduced MSCs engraft, survive, and express the therapeutic gene up to 6 weeks posttransplantation, while maintaining an undifferentiated phenotype. In conclusion, transplanted MSCs have limited capacity for the replacement of neural cells lost as a result of a spinal cord trauma. However, they provide excellent opportunities for local delivery of neurotrophic factors into the injured tissue. This study underlines the therapeutic benefits associated with cell transplantation and provides a good example of the use of MSCs for gene delivery.en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherMary Ann Lieberten_US
dc.relation.ispartofTissue Engineering Part Aen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectMarrow stromal cellsen_US
dc.subjectNerve growth factoren_US
dc.subjectBone marrowen_US
dc.subjectIn vitroen_US
dc.subjectAxonal growthen_US
dc.subjectAdult raten_US
dc.subjectBrainen_US
dc.subjectRecoveryen_US
dc.subjectInjuryen_US
dc.subjectGDNFen_US
dc.titleNeurotrophic Factor-Expressing Mesenchymal Stem Cells Survive Transplantation into the Contused Spinal Cord Without Differentiating into Neural Cellsen_US
dc.typeArticleen_US
dc.date.updated2014-02-24T22:36:15Z
dc.identifier.doiDOI 10.1089/ten.tea.2009.0045
dc.local.publishedsourcehttp://dx.doi.org/10.1089/ten.tea.2009.0045en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid1336310
dc.local.contactSiobhan Mcmahon, Department Of Anatomy, Nui, Galway. 2838 Email: siobhan.mcmahon@nuigalway.ie
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dc.local.versionACCEPTED
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