dc.contributor.author | Rooney, Gemma E. | |
dc.contributor.author | McMahon, Siobhan | |
dc.contributor.author | Ritter, Thomas | |
dc.contributor.author | Garcia, Yolanda | |
dc.contributor.author | Moran, Cathal | |
dc.contributor.author | Madigan, Nicholas N. | |
dc.date.accessioned | 2014-02-26T15:09:32Z | |
dc.date.available | 2014-02-26T15:09:32Z | |
dc.date.issued | 2009-10 | |
dc.identifier.citation | Rooney, GE,McMahon, SS,Ritter, T,Garcia, Y,Moran, C,Madigan, NN,Flugel, A,Dockery, P,O'Brien, T,Howard, L,Windebank, AJ,Barry, FP (2009) 'Neurotrophic Factor-Expressing Mesenchymal Stem Cells Survive Transplantation into the Contused Spinal Cord Without Differentiating into Neural Cells'. Tissue Engineering Part A, 15 :3049-3059. | en_US |
dc.identifier.uri | http://hdl.handle.net/10379/4226 | |
dc.description | Journal article | en_US |
dc.description.abstract | The aim of this study was to assess the feasibility of transplanting mesenchymal stem cells (MSCs), genetically modified to express glial-derived neurotrophic factor (GDNF), to the contused rat spinal cord, and to subsequently assess their neural differentiation potential. MSCs expressing green fluorescent protein were transduced with a retroviral vector to express the neurotrophin GDNF. The transduction protocol was optimized by using green fluorescent protein-expressing retroviral constructs; approximately 90% of MSCs were transduced successfully after G418 selection. GDNF-transduced MSCs expressed the transgene and secreted growth factor into the media (similar to 12 ng/500,000 cells secreted into the supernatant 2 weeks after transduction). Injuries were established using an impactor device, which applied a given, reproducible force to the exposed spinal cord. GDNF-expressing MSCs were transplanted rostral and caudal to the site of injury. Spinal cord sections were analyzed 2 and 6 weeks after transplantation. We demonstrate that GDNF-transduced MSCs engraft, survive, and express the therapeutic gene up to 6 weeks posttransplantation, while maintaining an undifferentiated phenotype. In conclusion, transplanted MSCs have limited capacity for the replacement of neural cells lost as a result of a spinal cord trauma. However, they provide excellent opportunities for local delivery of neurotrophic factors into the injured tissue. This study underlines the therapeutic benefits associated with cell transplantation and provides a good example of the use of MSCs for gene delivery. | en_US |
dc.format | application/pdf | en_US |
dc.language.iso | en | en_US |
dc.publisher | Mary Ann Liebert | en_US |
dc.relation.ispartof | Tissue Engineering Part A | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Marrow stromal cells | en_US |
dc.subject | Nerve growth factor | en_US |
dc.subject | Bone marrow | en_US |
dc.subject | In vitro | en_US |
dc.subject | Axonal growth | en_US |
dc.subject | Adult rat | en_US |
dc.subject | Brain | en_US |
dc.subject | Recovery | en_US |
dc.subject | Injury | en_US |
dc.subject | GDNF | en_US |
dc.title | Neurotrophic Factor-Expressing Mesenchymal Stem Cells Survive Transplantation into the Contused Spinal Cord Without Differentiating into Neural Cells | en_US |
dc.type | Article | en_US |
dc.date.updated | 2014-02-24T22:36:15Z | |
dc.identifier.doi | DOI 10.1089/ten.tea.2009.0045 | |
dc.local.publishedsource | http://dx.doi.org/10.1089/ten.tea.2009.0045 | en_US |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | |~| | |
dc.internal.rssid | 1336310 | |
dc.local.contact | Siobhan Mcmahon, Department Of Anatomy, Nui, Galway. 2838 Email: siobhan.mcmahon@nuigalway.ie | |
dc.local.copyrightchecked | No | |
dc.local.version | ACCEPTED | |
nui.item.downloads | 602 | |