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dc.contributor.authorFleming, Gerard T.
dc.contributor.authorPatching, John W.
dc.date.accessioned2014-01-27T14:32:20Z
dc.date.available2014-01-27T14:32:20Z
dc.date.issued1988-03-23
dc.identifier.citationFLEMING, G,DAWSON, MT,PATCHING, JW (1988) 'The isolation of strains of Bacillus subtilis showing improved plasmid stability characteristics by means of selective chemostat culture'. Journal Of General Microbiology, 134 :2095-2101.en_US
dc.identifier.issn1367-5435
dc.identifier.urihttp://hdl.handle.net/10379/4046
dc.descriptionJournal articleen_US
dc.description.abstractA PUB 110-derived plasmid encoding chloramphenicol resistance, kanamycin resistance and high-temperature alpha-amylase showed a high degree of segregational instability when inserted into Bacillus subtilis. In an attempt to obtain stable derivatives, the organism was grown in chemostat culture in the presence of chloramphenicol. It was periodically found necessary to increase the concentration of chloramphenicol in the medium feed in order to avoid plasmid loss. Strains were isolated after 19 and 160 generations, which showed high levels of plasmid stability. This characteristic appeared to be genotypic. No detectable difference in plasmid copy number was found between the original and the improved strains. The stability characteristics resided in the host, rather than in the plasmid. Stable isolates possessed elevated MICs for both chloramphenicol and kanamycin. Their maximum specific growth rates were higher than that of the original strain, and similar to that of the plasmid-free parent strain.en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.relation.ispartofJournal Of General Microbiologyen
dc.subjectBacillus subtilisen_US
dc.titleThe isolation of strains of Bacillus subtilis showing improved plasmid stability characteristics by means of selective chemostat cultureen_US
dc.typeArticleen_US
dc.date.updated2013-11-04T12:34:39Z
dc.local.publishedsourcehttp://www.ncbi.nlm.nih.gov/pubmed/3150976en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid1359427
dc.local.contactGerard Fleming, Dept. Of Microbiology, Arts/Science Building, Nui Galway. 3562 Email: ger.fleming@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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