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dc.contributor.authorDoheny, HC,Houlihan, DD,Ravikumar, N,Smith, TJ,Morrison, JJ
dc.date.accessioned2014-01-23T13:57:07Z
dc.date.available2014-01-23T13:57:07Z
dc.date.issued2003
dc.identifier.citationDoheny, HC,Houlihan, DD,Ravikumar, N,Smith, TJ,Morrison, JJ (2003) 'Human chorionic gonadotrophin relaxation of human pregnant myometrium and activation of the BKCa channel'. Journal Of Clinical Endocrinology & Metabolism, 88 :4310-4315.en
dc.identifier.urihttp://hdl.handle.net/10379/4016
dc.description.abstractThe uterorelaxant effect of human chorionic gonadotropin (hCG) is regarded as an important mediator in maintenance of uterine quiescence during pregnancy with clinical potential for tocolysis, the mechanisms of which are unknown. The large conductance calcium-activated K+ channel (BKCa) is ubiquitously encountered in human uterine tissue and plays a significant role in modulating myometrial cell membrane potential and excitability. The objective of this study was to investigate the involvement of BKCa channel function in the response of human myometrial cells to hCG. Single electrophysiological BKCa channel recordings from freshly dispersed myocytes were obtained in the presence and absence of increasing hCG concentrations. Isometric tension studies, investigating the effects of hCG on isolated myometrial contractions, in the presence and absence of the BKCa channel blocker, iberiotoxin, were performed. The hCG significantly increased the open-state probability of these channels in a concentration-dependent manner [control 0.036 +/- 0.01; 1 IU/ml hCG 0.065 +/- 0.014 (P +/- 0.262); 10 IU/ml hCG 0.111 +/- 0.009 (P = 0.001); and 100 IU/ml hCG 0.098 +/- 0.004 (P = 0.007)]. In vitro functional studies demonstrated that hCG exerted a significant concentration-dependent relaxant effect on human myometrial tissue. This effect was significantly attenuated by preincubation with iberiotoxin (Pen
dc.language.isoen
dc.relation.ispartofJournal Of Clinical Endocrinology & Metabolismen
dc.subjectCONDUCTANCE K+-CHANNELS
dc.subjectPOTASSIUM CHANNELS
dc.subjectPRETERM DELIVERY
dc.subjectCONTRACTILITY
dc.subjectCALCIUM
dc.subjectLABOR
dc.subjectINHIBITION
dc.subjectMYOCYTES
dc.titleHuman chorionic gonadotrophin relaxation of human pregnant myometrium and activation of the BKCa channelen
dc.typeArticle
dc.date.updated2013-10-07T15:34:10Z
dc.identifier.doiDOI 10.1210/jc.2003-030221
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid1057487
dc.local.contactTerry Smith, School Of Natural Sciences, Nui Galway. 5488 Email: terry.smith@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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