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dc.contributor.authorRyder, Alan G.
dc.contributor.authorLi, Boyan
dc.contributor.authorSirimithu, Narayana M.S.
dc.contributor.authorRay, Bryan H.
dc.date.accessioned2014-01-21T14:28:40Z
dc.date.available2014-01-21T14:28:40Z
dc.date.issued2012
dc.identifier.citationLi, BY,Sirimuthu, NMS,Ray, BH,Ryder, AG (2012) 'Using surface-enhanced Raman scattering (SERS) and fluorescence spectroscopy for screening yeast extracts, a complex component of cell culture media'. Journal Of Raman Spectroscopy, 43 :1074-1082.en_US
dc.identifier.urihttp://dx.doi.org/10.1002/jrs.3141
dc.identifier.urihttp://hdl.handle.net/10379/3982
dc.description.abstractYeastolate or yeast extract, which are hydrolysates produced by autolysis of yeast, are often employed as a raw material in the media used for industrial mammalian cell culture. The source and quality of yeastolate can significantly affect cell growth and production; however, analysis of these complex biologically derived materials is not straightforward. The best current method, liquid chromatographymass spectrometry (LC-MS), is time-consuming and requires extensive expertise. This study describes the use of surface-enhanced Raman scattering (SERS) and fluorescence excitationemission matrix (EEM) spectroscopy coupled with robust principal component analysis (ROBPCA) for the rapid and facile characterization and discrimination of yeast extracts in aqueous solution. SERS using silver colloids generates time-dependent signals, where adenine is the strongest contributor, and the spectra are stable and reproducible (< ~3%) at 180 minutes after mixing. Combining this spectral behavior with chemometric methods enables SERS to be used for discriminating between different yeastolate sources, for assessing lot-to-lot variability, and potentially to monitor storage-induced compositional changes. Fluorescence EEM combined with multiway ROBPCA also provides a rapid and inexpensive method for discrimination of yeastolate, yielding very similar results in terms of sample discrimination to that obtained by SERS. However, the EEM data does not provide the same level of chemical information as provided by the SERS. Thus the combination of the two methodologies has the potential to be extremely useful in biopharmaceutical manufacturing for the rapid characterization and screening of biogenic hydrolysates from animal or plant sources.en_US
dc.description.sponsorshipIrish Industrial Development Authorityen_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.relation.ispartofJournal Of Raman Spectroscopyen
dc.subjectSurface-enhanced Raman scattering spectroscopyen_US
dc.subjectFluorescence excitation-emission matrix spectroscopyen_US
dc.subjectYeastolateen_US
dc.subjectYeast extracten_US
dc.subjectRobust principal component analysisen_US
dc.subjectEEM spectroscopyen_US
dc.subjectSilver surfacesen_US
dc.subjectBacterial-cellsen_US
dc.subjectGrowth mediaen_US
dc.subjectSpectraen_US
dc.subjectIdentificationen_US
dc.subjectAdsorptionen_US
dc.subjectClassificationen_US
dc.subjectChemometricsen_US
dc.subjectDerivativesen_US
dc.titleUsing surface-enhanced Raman scattering (SERS) and fluorescence spectroscopy for screening yeast extracts, a complex component of cell culture mediaen_US
dc.typeArticleen_US
dc.date.updated2013-09-24T15:08:48Z
dc.identifier.doihttp://dx.doi.org/10.1002/jrs.3141
dc.local.publishedsourcehttp://dx.doi.org/10.1002/jrs.3141en_US
dc.local.publisherstatementThis is the author corrected version, the definitive version is published on the Journal of Raman Spectroscopy website.en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid2423880
dc.local.contactAlan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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