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dc.contributor.authorYang, Haixuan
dc.date.accessioned2013-11-28T12:35:33Z
dc.date.available2013-11-28T12:35:33Z
dc.date.issued2012-08-11
dc.identifier.citationHavugimana, Pierre C and Hart, G Traver and Nepusz, Tamas and Yang, Haixuan and Turinsky, Andrei L and Li, Zhihua and Wang, Peggy I and Boutz, Daniel R and Fong, Vincent and Phanse, Sadhna et al (2012) 'A census of human soluble protein complexes'. Cell, 150 (5):1068-1081.en_US
dc.identifier.issn0092-8674
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0092867412010069#
dc.identifier.urihttp://hdl.handle.net/10379/3852
dc.descriptionJournal articleen_US
dc.description.abstractCellular processes often depend on stable physical associations between proteins. Despite recent progress, knowledge of the composition of human protein complexes remains limited. To close this gap, we applied an integrative global proteomic profiling approach, based on chromatographic separation of cultured human cell extracts into more than one thousand biochemical fractions that were subsequently analyzed by quantitative tandem mass spectrometry, to systematically identify a network of 13,993 high-confidence physical interactions among 3,006 stably associated soluble human proteins. Most of the 622 putative protein complexes we report are linked to core biological processes and encompass both candidate disease genes and unannotated proteins to inform on mechanism. Strikingly, whereas larger multiprotein assemblies tend to be more extensively annotated and evolutionarily conserved, human protein complexes with five or fewer subunits are far more likely to be functionally unannotated or restricted to vertebrates, suggesting more recent functional innovations.en_US
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BB/F00964X/1 and BB/K004131/1); Royal Society (NF080750); Canada Institutes of Health Research (MOP 82940)en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofCellen
dc.subjectHuman cellsen_US
dc.subjectProteinsen_US
dc.titleA census of human soluble protein complexesen_US
dc.typeArticleen_US
dc.date.updated2013-09-24T16:16:00Z
dc.identifier.doi10.1016/j.cell.2012.08.011
dc.local.publishedsourcehttp://dx.doi.org/10.1016/j.cell.2012.08.011en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid3743215
dc.local.contactHaixuan Yang, School Of Mathematics,Statistics, & Applied Mathematics, Adb-G013, Nui Galway. 2320 Email: haixuan.yang@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionPUBLISHED
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