Cell cycle-dependent mobility of Cdc45 in living cells determined by fluorescence correlation spectroscopy.
|dc.contributor.author||Togashi, Denisio M.|
|dc.contributor.author||Ryder, Alan G.|
|dc.identifier.citation||R. Broderick, S. Ramadurai, K. Toth, D. Togashi, A. G. Ryder, J. Langwoski, and H.P. Nasheuer. (2012) 'Cell cycle-dependent mobility of Cdc45 in living cells determined by fluorescence correlation spectroscopy'. Plos One, 7 (4):e35537-e35537.||en_US|
|dc.description.abstract||Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our data show that eGFP-Cdc45 mobility is faster in G1/S transition compared to S phase suggesting that Cdc45 is part of larger protein complex formed in S phase. Furthermore, the size of complexes containing Cdc45 was estimated in asynchronous, G1/S and S phase-synchronized cells using gel filtration chromatography; these findings complemented the in vivo FCS data. Analysis of the mobility of eGFP-Cdc45 and the size ofcomplexes containing Cdc45 and eGFP-Cdc45 after UVC-mediated DNA damage revealed no significant changes in diffusion rates and complex sizes using FCS and gel filtration chromatography analyses. This suggests that after UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage.||en_US|
|dc.publisher||Public Library of Science||en_US|
|dc.subject||Eukaryotic DNA replication|
|dc.title||Cell cycle-dependent mobility of Cdc45 in living cells determined by fluorescence correlation spectroscopy.||en_US|
|dc.local.contact||Alan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: email@example.com|
Files in this item
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.
The following license files are associated with this item: