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dc.contributor.authorKliszczak, Anna E.
dc.contributor.authorRainey, Michael D.
dc.contributor.authorHarhen, Brendan
dc.contributor.authorSantocanale, Corrado
dc.date.accessioned2011-10-28T14:00:34Z
dc.date.available2011-10-28T14:00:34Z
dc.date.issued2011
dc.identifier.citationKliszczak, A. E., Rainey, M. D., Harhen, B., Boisvert, F. M., & Santocanale, C. (2011) DNA mediated chromatin pull-down for the study of chromatin replication. Sci. Rep., 1.en_US
dc.identifier.urihttp://hdl.handle.net/10379/2244
dc.description.abstractChromatin replication involves duplicating DNA while maintaining epigenetic information. These processes are critical for genome stability and for preserving cell-type identity. Here we describe a simple experimental approach that allows chromatin to be captured and its content analysed after in vivo replication and labeling of DNA by cellular DNA polymerases. We show that this technique is highly specific and that proteins bound to the replicated DNA can be analyzed by both immunological techniques and large scale mass spectrometry. As proof of concept we have used this novel procedure to begin investigating the relationship between chromatin protein composition and the temporal programme of DNA replication in human cells. It is expected that this technique will become a widely used tool to address how chromatin proteins assemble onto newly replicated DNA after passage of a replication fork and how chromatin maturation is coupled to DNA synthesis.en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectEpigeneticsen_US
dc.subjectDNA replicationen_US
dc.subjectProteomicsen_US
dc.subjectChromatinen_US
dc.subjectNational Centre of Biomedical Engineering and Scienceen_US
dc.titleDNA mediated chromatin pull-down for the study of chromatin replicationen_US
dc.typeArticleen_US
dc.local.publishedsourcehttp://dx.doi.org/10.1038/srep00095en_US
dc.description.peer-reviewedpeer-revieweden_US
dc.contributor.funderScience Foundation Irelanden_US
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland