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dc.contributor.authorHooban, Brigid
dc.contributor.authorFitzhenry, Kelly
dc.contributor.authorCahill, Niamh
dc.contributor.authorJoyce, Aoife
dc.contributor.authorO' Connor, Louise
dc.contributor.authorBray, James E.
dc.contributor.authorBrisse, Sylvain
dc.contributor.authorPasset, Virginie
dc.contributor.authorAbbas Syed, Raza
dc.contributor.authorCormican, Martin
dc.contributor.authorMorris, Dearbháile
dc.date.accessioned2021-03-25T11:57:49Z
dc.date.available2021-03-25T11:57:49Z
dc.date.issued2021-03-08
dc.identifier.citationHooban, Brigid, Fitzhenry, Kelly, Cahill, Niamh, Joyce, Aoife, O' Connor, Louise, Bray, James E., Brisse, Sylvain, Passet, Virginie, Abbas Syed, Raza, Cormican, Martin, Morris, Dearbháile, Morris, Dearbháile. (2021). A Point Prevalence Survey of Antibiotic Resistance in the Irish Environment, 2018–2019. Environment International, 152, doi:https://doi.org/10.1016/j.envint.2021.106466en_IE
dc.identifier.urihttp://hdl.handle.net/10379/16638
dc.description.abstractWater bodies worldwide have proven to be vast reservoirs of clinically significant antibiotic resistant organisms. Contamination of waters by anthropogenic discharges is a significant contributor to the widespread dissemination of antibiotic resistance. The aim of this research was to investigate multiple different anthropogenic sources on a national scale for the role they play in the environmental propagation of antibiotic resistance. A total of 39 water and 25 sewage samples were collected across four local authority areas in the West, East and South of Ireland. In total, 211 Enterobacterales were isolated (139 water, 72 sewage) and characterised. A subset of isolates (n=60) were chosen for whole genome sequencing. Direct comparisons of the water versus sewage isolate collections revealed a higher percentage of sewage isolates displayed resistance to cefoxitin (46%) and ertapenem (32%), while a higher percentage of water isolates displayed resistance to tetracycline (55%) and ciprofloxacin (71%). Half of all isolates displayed extended spectrum beta-lactamase (ESBL) production phenotypically (n = 105/211; 50%), with blaCTX-M detected in 99/105 isolates by PCR. Carbapenemase genes were identified in 11 isolates (6 sewage, 5 water). The most common variant was blaOXA-48 (n=6), followed by blaNDM-5 (n=2) and blaKPC-2 (n=2). Whole genome sequencing analysis revealed numerous different sequence types in circulation in both waters and sewage including E. coli ST131 (n=15), ST38 (n=8), ST10 (n=4) along with Klebsiella ST405 (n=3) and ST11 (n=2). Core genome MLST (cgMLST) comparisons uncovered three highly similar Klebsiella isolates originating from hospital sewage and two nearby waters. The Klebsiella isolates from an estuary and seawater displayed 99.1% and 98.8% cgMLST identity to the hospital sewage isolate respectively. In addition, three pairs of E. coli isolates from different waters also revealed cgMLST similarities, indicating widespread dissemination and persistence of certain strains in the aquatic environment. These findings highlight the need for routine monitoring of water bodies used for recreational and drinking purposes for the presence of multi-drug resistant organisms.en_IE
dc.description.sponsorshipThis project is jointly funded by the Environmental Protection agency, under the EPA Research Programme 2014-2020, and the Health Service Executive (2017-HW-LS-1). The EPA Research Programme is a Government of Ireland initiative funded by the Department of Communications, Climate Action and Environment. It is administered by the Environmental Protection Agency, which has the statutory function of co-ordinating and promoting environmental research. A portion of this work was also financially supported by the MedVetKlebs project, a component of the European Joint Programme One Health EJP, which has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under Grant Agreement No. 773830.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofEnvironment Internationalen
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAntibiotic resistanceen_IE
dc.subjectAquatic Environmenten_IE
dc.subjectWastewateren_IE
dc.subjectCarbapenemaseen_IE
dc.subjectExtended Spectrum Beta-Lactamaseen_IE
dc.titleA point prevalence survey of antibiotic resistance in the Irish environment, 2018-2019en_IE
dc.typeArticleen_IE
dc.date.updated2021-03-25T11:41:19Z
dc.identifier.doi10.1016/j.envint.2021.106466
dc.local.publishedsourcehttps://dx.doi.org/10.1016/j.envint.2021.106466en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderEnvironmental Protection Agency (EPA), Irelanden_IE
dc.contributor.funderHealth Service Executiveen_IE
dc.contributor.funderHorizon 2020 Framework Programmeen_IE
dc.internal.rssid25357374
dc.local.contactBrigid Hooban, Clinical Science Institute, , Shantalla, , Galway. Email: b.hooban1@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/EC/H2020::COFUND-EJP/773830/EU/Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards./One Health EJPen_IE
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Attribution 4.0 International (CC BY 4.0)
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