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dc.contributor.authorBroderick, Ronan
dc.contributor.authorRamadurai, Sivaramakrishnan
dc.contributor.authorTóth, Katalin
dc.contributor.authorTogashi, Denisio M.
dc.contributor.authorRyder, Alan G.
dc.contributor.authorLangowski, Jörg
dc.contributor.authorNasheuer, Heinz-Peter
dc.date.accessioned2021-01-07T09:45:29Z
dc.date.available2021-01-07T09:45:29Z
dc.date.issued2012-04-19
dc.identifier.citationBroderick R, Ramadurai S, Tóth K, Togashi DM, Ryder AG, Langowski J, et al. (2012) Cell Cycle-Dependent Mobility of Cdc45 Determined in vivo by Fluorescence Correlation Spectroscopy. PLoS ONE 7(4): e35537. https://doi.org/10.1371/journal.pone.0035537en_IE
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10379/16422
dc.description.abstractEukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our data show that eGFP-Cdc45 mobility is faster in G1/S transition compared to S phase suggesting that Cdc45 is part of larger protein complex formed in S phase. Furthermore, the size of complexes containing Cdc45 was estimated in asynchronous, G1/S and S phase-synchronized cells using gel filtration chromatography; these findings complemented the in vivo FCS data. Analysis of the mobility of eGFP-Cdc45 and the size of complexes containing Cdc45 and eGFP-Cdc45 after UVC-mediated DNA damage revealed no significant changes in diffusion rates and complex sizes using FCS and gel filtration chromatography analyses. This suggests that after UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherPublic Library of Scienceen_IE
dc.relation.ispartofPlos Oneen
dc.subjectEUKARYOTIC DNA-REPLICATIONen_IE
dc.subjectPROTEIN-Aen_IE
dc.subjectPOLYMERASE-ALPHAen_IE
dc.subjectMCM2-7 HELICASEen_IE
dc.subjectS-PHASEen_IE
dc.subjectCOMPLEXen_IE
dc.subjectINITIATIONen_IE
dc.subjectDYNAMICSen_IE
dc.subjectCHROMATINen_IE
dc.subjectGINSen_IE
dc.titleCell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopyen_IE
dc.typeArticleen_IE
dc.date.updated2021-01-05T21:40:06Z
dc.identifier.doi10.1371/journal.pone.0035537
dc.local.publishedsourcehttps://doi.org/10.1371/journal.pone.0035537en_IE
dc.description.peer-reviewedpeer-reviewed
dc.internal.rssid2194649
dc.local.contactHeinz-Peter Nasheuer, 2fn, Rm Bms 2010, Biochemistry S, Bioresearch Building, Nui Galway, Galway. 2430 Email: heinz.nasheuer@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
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