dc.contributor.author | Smith, David | |
dc.contributor.author | Cwiklinski, Krystyna | |
dc.contributor.author | Jewhurst, Heather | |
dc.contributor.author | Tikhonova, Irina G. | |
dc.contributor.author | Dalton, John P. | |
dc.date.accessioned | 2020-11-30T11:15:00Z | |
dc.date.available | 2020-11-30T11:15:00Z | |
dc.date.issued | 2020-11-26 | |
dc.identifier.citation | Smith, David, Cwiklinski, Krystyna, Jewhurst, Heather, Tikhonova, Irina G., & Dalton, John P. (2020). An atypical and functionally diverse family of Kunitz-type cysteine/serine proteinase inhibitors secreted by the helminth parasite Fasciola hepatica. Scientific Reports, 10(1), 20657. doi:10.1038/s41598-020-77687-7 | en_IE |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10379/16350 | |
dc.description.abstract | Fasciola hepatica is a global parasite of humans and their livestock. Regulation of parasite-secreted cathepsin L-like cysteine proteases associated with virulence is important to fine-tune parasite-host interaction. We uncovered a family of seven Kunitz-type (FhKT) inhibitors dispersed into five phylogenetic groups. The most highly expressed FhKT genes (group FhKT1) are secreted by the newly excysted juveniles (NEJs), the stage responsible for host infection. The FhKT1 inhibitors do not inhibit serine proteases but are potent inhibitors of parasite cathepsins L and host lysosomal cathepsin L, S and K cysteine proteases (inhibition constants¿ | en_IE |
dc.description.sponsorship | DS is funded by a Department for Employment and Learning (DEL), Northern Ireland grant. KC and JPD are funded by a European Research Council Advanced Grant (HELIVAC, 322725) and Science Foundation Ireland (SFI) Professorship grant (17/RP/5368). HJ, KC and JPD are members of the Horizon 2020-funded Consortium PARAGONE. | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | Nature Research | en_IE |
dc.relation.ispartof | Scientific Reports | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Biochemistry | en_IE |
dc.subject | Diseases | en_IE |
dc.subject | Molecular biology | en_IE |
dc.subject | Fasciola | en_IE |
dc.subject | Trematode | en_IE |
dc.subject | Cathepsin | en_IE |
dc.subject | Cysteine Protease | en_IE |
dc.subject | Kunitz | en_IE |
dc.subject | Serine Protease | en_IE |
dc.subject | Inhibitors | en_IE |
dc.title | An atypical and functionally diverse family of Kunitz-type cysteine/serine proteinase inhibitors secreted by the helminth parasite Fasciola hepatica. | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2020-11-30T10:40:05Z | |
dc.identifier.doi | 10.1038/s41598-020-77687-7 | |
dc.local.publishedsource | https://doi.org/10.1038/s41598-020-77687-7 | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | Department for Employment and Learning, Northern Ireland | en_IE |
dc.contributor.funder | European Research Council | en_IE |
dc.contributor.funder | Science Foundation Ireland | en_IE |
dc.contributor.funder | Horizon 2020 | en_IE |
dc.internal.rssid | 23732957 | |
dc.local.contact | Krystyna Cwiklinski. Email: krystyna.cwiklinski@nuigalway.ie | |
dc.local.copyrightchecked | Yes | |
dc.local.version | ACCEPTED | |
dcterms.project | info:eu-repo/grantAgreement/EC/FP7::SP2::ERC/322725/EU/Overpowering helminth-mediated immune-modulation is a route towards vaccine development against these major animal pathogens/HELIVAC | en_IE |
nui.item.downloads | 100 | |