Show simple item record

dc.contributor.authorSmith, David
dc.contributor.authorCwiklinski, Krystyna
dc.contributor.authorJewhurst, Heather
dc.contributor.authorTikhonova, Irina G.
dc.contributor.authorDalton, John P.
dc.date.accessioned2020-11-30T11:15:00Z
dc.date.available2020-11-30T11:15:00Z
dc.date.issued2020-11-26
dc.identifier.citationSmith, David, Cwiklinski, Krystyna, Jewhurst, Heather, Tikhonova, Irina G., & Dalton, John P. (2020). An atypical and functionally diverse family of Kunitz-type cysteine/serine proteinase inhibitors secreted by the helminth parasite Fasciola hepatica. Scientific Reports, 10(1), 20657. doi:10.1038/s41598-020-77687-7en_IE
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10379/16350
dc.description.abstractFasciola hepatica is a global parasite of humans and their livestock. Regulation of parasite-secreted cathepsin L-like cysteine proteases associated with virulence is important to fine-tune parasite-host interaction. We uncovered a family of seven Kunitz-type (FhKT) inhibitors dispersed into five phylogenetic groups. The most highly expressed FhKT genes (group FhKT1) are secreted by the newly excysted juveniles (NEJs), the stage responsible for host infection. The FhKT1 inhibitors do not inhibit serine proteases but are potent inhibitors of parasite cathepsins L and host lysosomal cathepsin L, S and K cysteine proteases (inhibition constants¿en_IE
dc.description.sponsorshipDS is funded by a Department for Employment and Learning (DEL), Northern Ireland grant. KC and JPD are funded by a European Research Council Advanced Grant (HELIVAC, 322725) and Science Foundation Ireland (SFI) Professorship grant (17/RP/5368). HJ, KC and JPD are members of the Horizon 2020-funded Consortium PARAGONE.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherNature Researchen_IE
dc.relation.ispartofScientific Reportsen
dc.subjectBiochemistryen_IE
dc.subjectDiseasesen_IE
dc.subjectMolecular biologyen_IE
dc.subjectFasciolaen_IE
dc.subjectTrematodeen_IE
dc.subjectCathepsinen_IE
dc.subjectCysteine Proteaseen_IE
dc.subjectKunitzen_IE
dc.subjectSerine Proteaseen_IE
dc.subjectInhibitorsen_IE
dc.titleAn atypical and functionally diverse family of Kunitz-type cysteine/serine proteinase inhibitors secreted by the helminth parasite Fasciola hepatica.en_IE
dc.typeArticleen_IE
dc.date.updated2020-11-30T10:40:05Z
dc.identifier.doi10.1038/s41598-020-77687-7
dc.local.publishedsourcehttps://doi.org/10.1038/s41598-020-77687-7en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderDepartment for Employment and Learning, Northern Irelanden_IE
dc.contributor.funderEuropean Research Councilen_IE
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderHorizon 2020en_IE
dc.internal.rssid23732957
dc.local.contactKrystyna Cwiklinski. Email: krystyna.cwiklinski@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/EC/FP7::SP2::ERC/322725/EU/Overpowering helminth-mediated immune-modulation is a route towards vaccine development against these major animal pathogens/HELIVACen_IE
nui.item.downloads37


Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:

Thumbnail

This item appears in the following Collection(s)

Show simple item record