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dc.contributor.authorTsiapalis, Dimitrios
dc.contributor.authorRana, Shubhasmin
dc.contributor.authorDoulgkeroglo, Meletios
dc.contributor.authorKearns, Stephen
dc.contributor.authorKelly, Jack
dc.contributor.authorBayon, Yves
dc.contributor.authorZeugolis, Dimitrios I.
dc.identifier.citationTsiapalis, Dimitrios, Rana, Shubhasmin, Doulgkeroglou, Meletios, Kearns, Stephen, Kelly, Jack, Bayon, Yves, & Zeugolis, Dimitrios I. (2020). Chapter 11 - The effect of aligned electrospun fibers and macromolecular crowding in tenocyte culture. In David Caballero, Subhas C. Kundu, & Rui L. Reis (Eds.), Methods in Cell Biology (Vol. 157, pp. 225-247): Academic Press.en_IE
dc.description.abstractTendon injuries continuously rise, and regeneration is not only slow, but also limited due to the poor endogenous healing ability of the tendon tissue. Tissue grafts constitute the clinical gold standard treatment for severe injuries, but inherent limitations drive the field toward tissue engineering approaches to create suitable tissue constructs. Recapitulation of the native microenvironment represent a key challenge for the development of tendon tissue equivalents in vitro that can be further utilized as implantable devices. Methods to maintain cellular phenotype and to enhance extracellular matrix deposition for accelerated development of tissue-like modulus should be developed. Herein, we assessed the combining effect of surface topography and macromolecular crowding in human tenocyte culture. Our data demonstrated that bidirectionally aligned electrospun fibers induce physiological cell growth, while macromolecular crowding enhanced and accelerated tissue-specific extracellular matrix deposition. Collectively, these data advocate the use of multifactorial approaches for the accelerated development of functional tissue-like surrogates in vitro.en_IE
dc.description.sponsorshipThe authors received financial support for this work from Science Foundation Ireland, Career Development Award (Grant Agreement Number: 15/CDA/3629); Science Foundation Ireland / European Regional Development Fund (Grant Agreement Number: 13/RC/2073); H2020, Marie Skłodowska-Curie Actions, Innovative Training Networks 2015 Tendon Therapy Train project (Grant Agreement Number: 676338).en_IE
dc.publisherElsevier Academic Pressen_IE
dc.relation.ispartofMethods in Cell Biologyen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjectCell therapiesen_IE
dc.subjectTendon tissue engineeringen_IE
dc.subjectIn vitro microenvironmenten_IE
dc.subjectMacromolecular crowdingen_IE
dc.subjectExtracellular matrix depositionen_IE
dc.titleThe effect of aligned electrospun fibers and macromolecular crowding in tenocyte cultureen_IE
dc.typeBook chapteren_IE
dc.description.peer-reviewedPeer reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderEuropean Regional Development Funden_IE
dc.contributor.funderHorizon 2020en_IE
dc.local.contactDimitros Tsiapalis, Remodel, Centre For Research In Medical Devices , Biomedical Sciences Building, Nui Galway. Email:
dc.local.copyrightcheckedYes - permission from publisher (see email from Dimitrios Tsiapalis on 03/06/2020)
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Career Development Award/15/CDA/3629/IE/Tissue Engineered Nanoassemblies _ Advanced Biomimicry of Living Equivalents (Short Title: TENABLE)/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/13/RC/2073/IE/C�RAM - Centre for Research in Medical Devices/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-ITN-ETN/676338/EU/Engineering in vitro microenvironments for translation of cell-based therapies for tendon repair/Tendon Therapy Trainen_IE

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