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dc.contributor.authorMarchese, Pietro
dc.contributor.authorGarzolib, Laura
dc.contributor.authorGnavib, Giorgio
dc.contributor.authorO’Connell, Enda
dc.contributor.authorBouraouid, Abderrahman
dc.contributor.authorMehirie, Mohamed
dc.contributor.authorMurphy, Mary
dc.contributor.authorVarese, Giovanna Cristina
dc.identifier.citationMarchese, Pietro, Garzolib, Laura, Gnavib, Giorgio, O’Connell, Enda, Bouraouid, Abderrahman, Mehirie, Mohamed, Murphy, Mary, Varese, Giovanna Cristina. (2020). Diversity and bioactivity of fungi associated with the marine sea cucumber Holothuria poli: disclosing the strains potential for biomedical applications. Journal of Applied Microbiology. doi:10.1111/jam.14659en_IE
dc.description.abstractAims: Identification of the mycobiota associated to the marine echinoderm Holothuria poli and investigation of cytotoxic and pro-osteogenic potential of isolated strains. Methods and results: Fungal strains were isolated from the animal s body- wall, intestine and faeces. The species identification was based on DNA barcoding and morphophysiological observations. Forty-seven species were identified, all are Ascomycota and mainly belonging to Aspergillus and Penicillium genera. Sixteen strains were grown on three media for chemical extraction. Cytotoxic activity was tested on a hepatic cancer cell line (HepG2), the cells viability was evaluated after treatment using a resazurin based assay (AlamarBlue). Pro-osteogenic activity was tested on human Mesenchymal stem cell, differentiation was measured as the alkaline phosphatase production through reaction with p-nitrophenylphosphate or as the cells ability to mineralize calcium using a colorimetric kit (StanBio). Cytotoxic activity was recorded for four fungal species while five of 48 extracts highlighted bioactivity towards human mesenchymal stem cells. Conclusions: The presence of relevant animal-associated mycobiota was observed in H. poli and selected strains showed cytotoxic potential and pro- osteogenic activity. Significance and Impact of the Study: Our work represents the first report of a Mediterranean Sea cucumber mycobiota and highlights the isolates potential to synthetize compounds of pharmaceutical interest for regenerative medicine.en_IE
dc.description.sponsorshipWe acknowledge grant support from CRT Foundation, Science Foundation Ireland - Strategic Research Cluster 09/SRC/B1794 and Irish Research Council Postgraduate Scholarship GOIPG/2016/1113. The authors acknowledge the facilities and scientific and technical assistance of the Genomics and Screening Core at the National University of Ireland Galway, a facility that is funded by NUIG and the Irish Government’s Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007-2013.en_IE
dc.publisherSociety for Applied Microbiology (SfAM)en_IE
dc.relation.ispartofJournal Of Applied Microbiologyen
dc.subjectenvironmental mycologyen_IE
dc.subjectmarine fungien_IE
dc.subjectMediterranean Seaen_IE
dc.subjectsea cucumber.en_IE
dc.titleDiversity and bioactivity of fungi associated with the marine sea cucumber Holothuria poli: disclosing the strains potential for biomedical applicationsen_IE
dc.contributor.funderFondazione CRTen_IE
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderIrish Research Councilen_IE
dc.local.contactPietro Marchese, Biomedical Sciences Building, , Dangan, , Galway. Email:
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Strategic Research Cluster/09/SRC/B1794/IE/SRC REMEDI: REMEDI-2: From Research to Human Therapy/en_IE

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