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dc.contributor.authorMarchese, Pietro
dc.contributor.authorGarzolib, Laura
dc.contributor.authorGnavib, Giorgio
dc.contributor.authorO’Connell, Enda
dc.contributor.authorBouraouid, Abderrahman
dc.contributor.authorMehirie, Mohamed
dc.contributor.authorMurphy, Mary
dc.contributor.authorVarese, Giovanna Cristina
dc.date.accessioned2020-06-02T11:21:50Z
dc.date.issued2020-04-10
dc.identifier.citationMarchese, Pietro, Garzolib, Laura, Gnavib, Giorgio, O’Connell, Enda, Bouraouid, Abderrahman, Mehirie, Mohamed, Murphy, Mary, Varese, Giovanna Cristina. (2020). Diversity and bioactivity of fungi associated with the marine sea cucumber Holothuria poli: disclosing the strains potential for biomedical applications. Journal of Applied Microbiology. doi:10.1111/jam.14659en_IE
dc.identifier.issn1365-2672
dc.identifier.urihttp://hdl.handle.net/10379/15997
dc.description.abstractAims: Identification of the mycobiota associated to the marine echinoderm Holothuria poli and investigation of cytotoxic and pro-osteogenic potential of isolated strains. Methods and results: Fungal strains were isolated from the animal s body- wall, intestine and faeces. The species identification was based on DNA barcoding and morphophysiological observations. Forty-seven species were identified, all are Ascomycota and mainly belonging to Aspergillus and Penicillium genera. Sixteen strains were grown on three media for chemical extraction. Cytotoxic activity was tested on a hepatic cancer cell line (HepG2), the cells viability was evaluated after treatment using a resazurin based assay (AlamarBlue). Pro-osteogenic activity was tested on human Mesenchymal stem cell, differentiation was measured as the alkaline phosphatase production through reaction with p-nitrophenylphosphate or as the cells ability to mineralize calcium using a colorimetric kit (StanBio). Cytotoxic activity was recorded for four fungal species while five of 48 extracts highlighted bioactivity towards human mesenchymal stem cells. Conclusions: The presence of relevant animal-associated mycobiota was observed in H. poli and selected strains showed cytotoxic potential and pro- osteogenic activity. Significance and Impact of the Study: Our work represents the first report of a Mediterranean Sea cucumber mycobiota and highlights the isolates potential to synthetize compounds of pharmaceutical interest for regenerative medicine.en_IE
dc.description.sponsorshipWe acknowledge grant support from CRT Foundation, Science Foundation Ireland - Strategic Research Cluster 09/SRC/B1794 and Irish Research Council Postgraduate Scholarship GOIPG/2016/1113. The authors acknowledge the facilities and scientific and technical assistance of the Genomics and Screening Core at the National University of Ireland Galway, a facility that is funded by NUIG and the Irish Government’s Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007-2013.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherSociety for Applied Microbiology (SfAM)en_IE
dc.relation.ispartofJournal Of Applied Microbiologyen
dc.subjectcytotoxicityen_IE
dc.subjectenvironmental mycologyen_IE
dc.subjectmarine fungien_IE
dc.subjectMediterranean Seaen_IE
dc.subjectpharmaceuticalsen_IE
dc.subjectsea cucumber.en_IE
dc.titleDiversity and bioactivity of fungi associated with the marine sea cucumber Holothuria poli: disclosing the strains potential for biomedical applicationsen_IE
dc.typeArticleen_IE
dc.date.updated2020-05-29T09:05:50Z
dc.identifier.doi10.1111/jam.14659
dc.local.publishedsourcehttps://doi.org/10.1111/jam.14659en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderFondazione CRTen_IE
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderIrish Research Councilen_IE
dc.description.embargo2021-04-10
dc.internal.rssid21225699
dc.local.contactPietro Marchese, Biomedical Sciences Building, , Dangan, , Galway. Email: p.marchese1@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Strategic Research Cluster/09/SRC/B1794/IE/SRC REMEDI: REMEDI-2: From Research to Human Therapy/en_IE
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