dc.contributor.author | Marchese, Pietro | |
dc.contributor.author | Garzolib, Laura | |
dc.contributor.author | Gnavib, Giorgio | |
dc.contributor.author | O’Connell, Enda | |
dc.contributor.author | Bouraouid, Abderrahman | |
dc.contributor.author | Mehirie, Mohamed | |
dc.contributor.author | Murphy, Mary | |
dc.contributor.author | Varese, Giovanna Cristina | |
dc.date.accessioned | 2020-06-02T11:21:50Z | |
dc.date.issued | 2020-04-10 | |
dc.identifier.citation | Marchese, Pietro, Garzolib, Laura, Gnavib, Giorgio, O’Connell, Enda, Bouraouid, Abderrahman, Mehirie, Mohamed, Murphy, Mary, Varese, Giovanna Cristina. (2020). Diversity and bioactivity of fungi associated with the marine sea cucumber Holothuria poli: disclosing the strains potential for biomedical applications. Journal of Applied Microbiology. doi:10.1111/jam.14659 | en_IE |
dc.identifier.issn | 1365-2672 | |
dc.identifier.uri | http://hdl.handle.net/10379/15997 | |
dc.description.abstract | Aims: Identification of the mycobiota associated to the marine echinoderm Holothuria poli and investigation of cytotoxic and pro-osteogenic potential of isolated strains.
Methods and results: Fungal strains were isolated from the animal s body- wall, intestine and faeces. The species identification was based on DNA barcoding and morphophysiological observations. Forty-seven species were identified, all are Ascomycota and mainly belonging to Aspergillus and Penicillium genera. Sixteen strains were grown on three media for chemical extraction. Cytotoxic activity was tested on a hepatic cancer cell line (HepG2), the cells viability was evaluated after treatment using a resazurin based assay (AlamarBlue). Pro-osteogenic activity was tested on human Mesenchymal stem cell, differentiation was measured as the alkaline phosphatase production through reaction with p-nitrophenylphosphate or as the cells ability to mineralize calcium using a colorimetric kit (StanBio). Cytotoxic activity was recorded for four fungal species while five of 48 extracts highlighted bioactivity towards human mesenchymal stem cells.
Conclusions: The presence of relevant animal-associated mycobiota was observed in H. poli and selected strains showed cytotoxic potential and pro- osteogenic activity.
Significance and Impact of the Study: Our work represents the first report of a Mediterranean Sea cucumber mycobiota and highlights the isolates potential to synthetize compounds of pharmaceutical interest for regenerative medicine. | en_IE |
dc.description.sponsorship | We acknowledge grant support from CRT Foundation, Science Foundation
Ireland - Strategic Research Cluster 09/SRC/B1794 and Irish Research Council
Postgraduate Scholarship GOIPG/2016/1113. The authors acknowledge the
facilities and scientific and technical assistance of the Genomics and Screening
Core at the National University of Ireland Galway, a facility that is funded by
NUIG and the Irish Government’s Programme for Research in Third Level
Institutions, Cycles 4 and 5, National Development Plan 2007-2013. | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | Society for Applied Microbiology (SfAM) | en_IE |
dc.relation.ispartof | Journal Of Applied Microbiology | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | cytotoxicity | en_IE |
dc.subject | environmental mycology | en_IE |
dc.subject | marine fungi | en_IE |
dc.subject | Mediterranean Sea | en_IE |
dc.subject | pharmaceuticals | en_IE |
dc.subject | sea cucumber. | en_IE |
dc.title | Diversity and bioactivity of fungi associated with the marine sea cucumber Holothuria poli: disclosing the strains potential for biomedical applications | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2020-05-29T09:05:50Z | |
dc.identifier.doi | 10.1111/jam.14659 | |
dc.local.publishedsource | https://doi.org/10.1111/jam.14659 | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | Fondazione CRT | en_IE |
dc.contributor.funder | Science Foundation Ireland | en_IE |
dc.contributor.funder | Irish Research Council | en_IE |
dc.description.embargo | 2021-04-10 | |
dc.internal.rssid | 21225699 | |
dc.local.contact | Pietro Marchese, Biomedical Sciences Building, , Dangan, , Galway. Email: p.marchese1@nuigalway.ie | |
dc.local.copyrightchecked | Yes | |
dc.local.version | ACCEPTED | |
dcterms.project | info:eu-repo/grantAgreement/SFI/SFI Strategic Research Cluster/09/SRC/B1794/IE/SRC REMEDI: REMEDI-2: From Research to Human Therapy/ | en_IE |
nui.item.downloads | 253 | |