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dc.contributor.authorPalama, Maria Elisabetta Federica
dc.contributor.authorShaw, Georgina Margaret
dc.contributor.authorCarluccio, Simonetta
dc.contributor.authorReverberi, Daniele
dc.contributor.authorSercia, Laura
dc.contributor.authorPersano, Luana
dc.contributor.authorPisignano, Dario
dc.contributor.authorCortese, Katia
dc.contributor.authorBarry, Francis Peter
dc.contributor.authorMurphy, Josephine Mary
dc.contributor.authorGentili, Chiara
dc.date.accessioned2020-05-11T13:12:01Z
dc.date.available2020-05-11T13:12:01Z
dc.date.issued2020-02-14
dc.identifier.citationPalamà, Maria Elisabetta Federica, Shaw, Georgina Margaret, Carluccio, Simonetta, Reverberi, Daniele, Sercia, Laura, Persano, Luana, Pisignano,Dario, Cortese,Katia, Barry,Francis Peter, Murphy,Josephine Mary, Gentili, Chiara. (2020). The Secretome Derived From Mesenchymal Stromal Cells Cultured in a Xeno-Free Medium Promotes Human Cartilage Recovery in vitro. Frontiers in Bioengineering and Biotechnology, 8(90). doi:10.3389/fbioe.2020.00090en_IE
dc.identifier.issn2296-4185
dc.identifier.urihttp://hdl.handle.net/10379/15959
dc.description.abstractOsteoarthritis (OA) is a disabling joint disorder causing articular cartilage degeneration. Currently, the treatments are mainly aimed to pain and symptoms relief, rather than disease amelioration. Human bone marrow stromal cells (hBMSCs) have emerged as a promising paracrine mechanism-based tool for OA treatment. Here, we investigate the therapeutic potential of conditioned media (CM) and extracellular vesicles (EVs) isolated from hBMSC and grown in a xeno-free culture system (XFS) compared to the conventional fetal bovine serum-culture system (FBS) in an in vitro model of OA. First, we observed that XFS promoted growth and viability of hBMSCs compared to FBS-containing medium while preserving their typical phenotype. The biological effects of the CM derived from hBMSC cultivated in XFS- and FBS-based medium were tested on IL-1 alpha treated human chondrocytes, to mimic the OA enviroment. Treatment with CM derived from XFS-cultured hBMSC inhibited IL-1 alpha-induced expression of IL-6, IL-8, and COX-2 by hACs compared to FBS-based condition. Furthermore, we observed that hBMSCs grown in XFS produced a higher amount of EVs compared to FBS-culture. The hBMSC-EVs not only inhibit the adverse effects of IL-1 alpha-induced inflammation, but play a significant in vitro chondroprotective effect. In conclusion, the XFS medium was found to be suitable for isolation and expansion of hBMSCs with increased safety profile and intended for ready-to-use clinical therapies.en_IE
dc.description.sponsorshipThis work was received funding from the European Union’s Horizon 2020 Research and Innovation Program under grant agreement AUTOSTEM number 667932, the Science Foundation Ireland grant number 13/TIDA/B2668, and the FP7 European Union Health Collaborative Project PurStem, grant number 223298.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherFrontiers Mediaen_IE
dc.relation.ispartofFrontiers in Bioengineering and Biotechnologyen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectSTEM-CELLSen_IE
dc.subjectARTICULAR-CARTILAGEen_IE
dc.subjectMATRIX METALLOPROTEINASESen_IE
dc.subjectEXTRACELLULAR-MATRIXen_IE
dc.subjectKAPPA-Ben_IE
dc.subjectOSTEOARTHRITISen_IE
dc.subjectARTHRITISen_IE
dc.subjectBONEen_IE
dc.subjectDIFFERENTIATIONen_IE
dc.subjectEXPRESSIONen_IE
dc.titleThe secretome derived from mesenchymal stromal cells cultured in a xeno-free medium promotes human cartilage recovery in vitroen_IE
dc.typeArticleen_IE
dc.date.updated2020-05-11T12:24:14Z
dc.identifier.doi10.3389/fbioe.2020.00090
dc.local.publishedsourcehttps://doi.org/10.3389/fbioe.2020.00090en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderHorizon 2020en_IE
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderSeventh Framework Programmeen_IE
dc.internal.rssid20273696
dc.local.contactMary Murphy, Dept. Of Medicine & Remedi, Bms 1024, Biomedical Sciences Building,, North Campus. 5206 Email: mary.murphy@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/EC/H2020::RIA/667932/EU/Development of closed, scalable and AUTOmated robotic systems for therapeutic STEM cell manufacturing: aseptic systems from “Donor-to-Patient”/AUTOSTEMen_IE
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA) - Training Award/13/TIDA/B2668 TIDA Training 2014/IE/Validation of a novel serum-free medium for production of human mesenchymal stem cells and establishment of in vivo efficacy in a bone model of repair/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::HEALTH/223298/EU/Utilisation of the mesenchymal stem cell receptome for rational development of uniform, serum-free culture conditions and tools for cell characterization/PURSTEMen_IE
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland