dc.contributor.author | Djalali-Cuevas, Adrian | |
dc.contributor.author | Garnica-Galvez, Sergio | |
dc.contributor.author | Rampin, Andrea | |
dc.contributor.author | Gaspar, Diana | |
dc.contributor.author | Skoufos, Ioannis | |
dc.contributor.author | Tzora, Athina | |
dc.contributor.author | Prassinos, Nikitas | |
dc.contributor.author | Diakakis, Nikolaos | |
dc.contributor.author | Zeugolis, Dimitrios I. | |
dc.date.accessioned | 2020-04-22T09:55:14Z | |
dc.date.issued | 2019-03 | |
dc.identifier.citation | Djalali-Cuevas, Adrian, Garnica-Galvez, Sergio, Rampin, Andrea, Gaspar, Diana, Skoufos, Ioannis, Tzora, Athina, Prassinos, Nikitas, Diakakis, Nikolaos, Zeugolis, Dimitrios I. (2019). Preparation and Characterization of Tissue Surrogates Rich in Extracellular Matrix Using the Principles of Macromolecular Crowding. In: Vigetti D., Theocharis A.D. (eds) The Extracellular Matrix. Methods in Molecular Biology, vol 1952, doi:10.1007/978-1-4939-9133-4_20 | en_IE |
dc.identifier.issn | 1940-6029 | |
dc.identifier.uri | http://hdl.handle.net/10379/15901 | |
dc.description.abstract | Tissue engineering by self-assembly allows for the fabrication of living tissue surrogates by taking advantage of the cell's inherent ability to produce and deposit tissue-specific extracellular matrix. However, the long culture periods required to build a tissue substitute in conducive to phenotypic drift in vitro microenvironments result in phenotype and function losses. Although several biophysical microenvironmental modulators (e.g., surface topography, substrate stiffness, mechanical stimulation) have been used to address these issues, slow extracellular matrix deposition remains a limiting factor in clinical translation and commercialization of such therapies. Macromolecular crowding is an alternative in vitro microenvironment modulator that has been shown to accelerate extracellular matrix deposition by several orders of magnitude, thereby decreasing culture periods required for the development of an implantable device, while maintaining cell phenotype and function. Herein, we provide protocols for the production of tissue surrogates rich in extracellular matrix from human dermal fibroblasts, equine tenocytes, and equine adipose-derived stem cells using the principles of macromolecular crowding and the subsequent characterization thereof by means of immunofluorescent staining and complementary fluorescence intensity analysis. | en_IE |
dc.description.sponsorship | This work has been supported from the European Union, H2020 Research and Innovation Programme, ITN award, Tendon Therapy Train Project (grant agreement number: 676338); Science Foundation Ireland, Career Development Award Programme (grant agreement number: 15/CDA/3629); and Science Foundation Ireland and the European Regional Development Fund (grant agreement number: 13/RC/2073). The authors have no competing interests. | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | Humana Press | en_IE |
dc.relation.ispartof | Methods in Molecular Biology | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Macromolecular crowding | en_IE |
dc.subject | Excluding volume effect | en_IE |
dc.subject | Cell therapies | en_IE |
dc.subject | Immunocytochemistry | en_IE |
dc.subject | Extracellular matrix | en_IE |
dc.title | Preparation and characterization of tissue surrogates rich in extracellular matrix using the principles of macromolecular crowding | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2020-04-21T08:36:42Z | |
dc.identifier.doi | 10.1007/978-1-4939-9133-4_20 | |
dc.local.publishedsource | https://doi.org/10.1007/978-1-4939-9133-4_20 | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | Horizon 2020 | en_IE |
dc.contributor.funder | Science Foundation Ireland | en_IE |
dc.contributor.funder | European Regional Development Fund | en_IE |
dc.internal.rssid | 20707053 | |
dc.local.contact | Dimitrios Zeugolis, Mechanical & Biomedical Eng, Eng-2022, New Engineering Building, Nui Galway. 3166 Email: dimitrios.zeugolis@nuigalway.ie | |
dc.local.copyrightchecked | Yes | |
dc.local.version | ACCEPTED | |
dcterms.project | info:eu-repo/grantAgreement/EC/H2020::MSCA-ITN-ETN/676338/EU/Engineering in vitro microenvironments for translation of cell-based therapies for tendon repair/Tendon Therapy Train | en_IE |
dcterms.project | info:eu-repo/grantAgreement/SFI/SFI Career Development Award/15/CDA/3629/IE/Tissue Engineered Nanoassemblies _ Advanced Biomimicry of Living Equivalents (Short Title: TENABLE)/ | en_IE |
dcterms.project | info:eu-repo/grantAgreement/SFI/SFI Research Centres/13/RC/2073/IE/C�RAM - Centre for Research in Medical Devices/ | en_IE |
nui.item.downloads | 292 | |