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dc.contributor.authorFlannery, Andrea
dc.contributor.authorLe Berre, Marie
dc.contributor.authorPier, Gerald B.
dc.contributor.authorO’Gara, James P.
dc.contributor.authorKilcoyne, Michelle
dc.date.accessioned2020-04-06T11:29:48Z
dc.date.available2020-04-06T11:29:48Z
dc.date.issued2020-04-02
dc.identifier.citationFlannery, Andrea, Le Berre, Marie, Pier, Gerald B., O’Gara, James P., & Kilcoyne, Michelle. (2020). Glycomics Microarrays Reveal Differential In Situ Presentation of the Biofilm Polysaccharide Poly-N-acetylglucosamine on Acinetobacter baumannii and Staphylococcus aureus Cell Surfaces. 21(7), 2465, doi:10.3390/ijms21072465en_IE
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10379/15868
dc.description.abstractThe biofilm component poly-N-acetylglucosamine (PNAG) is an important virulence determinant in medical-device-related infections caused by ESKAPE group pathogens including Gram-positive Staphylococcus aureus and Gram-negative Acinetobacter baumannii. PNAG presentation on bacterial cell surfaces and its accessibility for host interactions are not fully understood. We employed a lectin microarray to examine PNAG surface presentation and interactions on methicillin-sensitive (MSSA) and methicillin-resistant S. aureus (MRSA) and a clinical A. baumannii isolate. Purified PNAG bound to wheatgerm agglutinin (WGA) and succinylated WGA (sWGA) lectins only. PNAG was the main accessible surface component on MSSA but was relatively inaccessible on the A. baumannii surface, where it modulated the presentation of other surface molecules. Carbohydrate microarrays demonstrated similar specificities of S. aureus and A. baumannii for their most intensely binding carbohydrates, including 3′ and 6′sialyllactose, but differences in moderately binding ligands, including blood groups A and B. An N-acetylglucosamine-binding lectin function which binds to PNAG identified on the A. baumannii cell surface may contribute to biofilm structure and PNAG surface presentation on A. baumannii. Overall, these data indicated differences in PNAG presentation and accessibility for interactions on Gram-positive and Gram-negative cell surfaces which may play an important role in biofilm-mediated pathogenesis.en_IE
dc.description.sponsorshipThis research was funded by the Irish Research Council for the Government of Ireland Postgraduate Scholarship award (A.F.), the Royal Society of Chemistry Analytical Chemistry Trust Fund (ACTF) for the 2018 ACTF Fellowship award (M.K.) and the Health Research Board (HRA-POR-2015-1158, J.P.O’G.).en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherMDPIen_IE
dc.relation.ispartofInternational Journal Of Molecular Sciencesen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectbiofilmen_IE
dc.subjectglycomics microarraysen_IE
dc.subjectbacterial adhesinsen_IE
dc.subjectpolysaccharideen_IE
dc.subjectStaphylococcus aureusen_IE
dc.subjectAcinetobacter baumannien_IE
dc.subjectpoly-N-acetylglucosamineen_IE
dc.subjectPNAGen_IE
dc.subjectlectinen_IE
dc.titleGlycomics microarrays reveal differential in situ presentation of the biofilm polysaccharide Poly-N-acetylglucosamine on Acinetobacter baumannii and Staphylococcus aureus cell surfacesen_IE
dc.typeArticleen_IE
dc.date.updated2020-04-06T11:17:51Z
dc.identifier.doi10.3390/ijms21072465
dc.local.publishedsourcehttps://doi.org/10.3390/ijms21072465en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderIrish Research Councilen_IE
dc.contributor.funderRoyal Society of Chemistry Analytical Chemistry Trust Funden_IE
dc.contributor.funderHealth Research Boarden_IE
dc.internal.rssid20520327
dc.local.contactMichelle Kilcoyne, Glycosciences, School Of Natural Sciences, Nui Galway. 5885 Email: michelle.kilcoyne@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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