RNA from a simple-tandem repeat is required for sperm maturation and male fertility in Drosophila melanogaster
View/ Open
Date
2019-11-05Author
Mills, Wilbur Kyle
Lee, Yuh Chwen G.
Kochendoerfer, Antje M.
Dunleavy, Elaine M.
Karpen, Gary H.
Metadata
Show full item recordUsage
This item's downloads: 288 (view details)
Cited 13 times in Scopus (view citations)
Recommended Citation
Mills, Wilbur Kyle, Lee, Yuh Chwen G., Kochendoerfer, Antje M., Dunleavy, Elaine M., & Karpen, Gary H. (2019). RNA from a simple-tandem repeat is required for sperm maturation and male fertility in Drosophila melanogaster. eLife, 8. doi: 10.7554/eLife.48940
Published Version
Abstract
Tandemly-repeated DNAs, or satellites, are enriched in heterochromatic regions of eukaryotic genomes and contribute to nuclear structure and function. Some satellites are transcribed, but we lack direct evidence that specific satellite RNAs are required for normal organismal functions. Here, we show satellite RNAs derived from AAGAG tandem repeats are transcribed in many cells throughout Drosophila melanogaster development, enriched in neurons and testes, often localized within heterochromatic regions, and important for viability. Strikingly, we find AAGAG transcripts are necessary for male fertility, and that AAGAG RNA depletion results in defective histone-protamine exchange, sperm maturation and chromatin organization. Since these events happen late in spermatogenesis when the transcripts are not detected, we speculate that AAGAG RNA in primary spermatocytes 'primes' post-meiosis steps for sperm maturation. In addition to demonstrating essential functions for AAGAG RNAs, comparisons between closely related Drosophila species suggest that satellites and their transcription evolve quickly to generate new functions.