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dc.contributor.authorHoueix, Benoit
dc.contributor.authorSynowsky, Silvia
dc.contributor.authorCairns, Michael T.
dc.contributor.authorKane, Marian
dc.contributor.authorKilcoyne, Michelle
dc.contributor.authorJoshi, Lokesh
dc.date.accessioned2020-01-03T09:51:21Z
dc.date.issued2019-11-11
dc.identifier.citationHoueix, Benoit, Synowsky, Silvia, Cairns, Michael T, Kane, Marian, Kilcoyne, Michelle, & Joshi, Lokesh. (2019). Identification of putative adhesins and carbohydrate ligands of Lactobacillus paracasei using a combinatorial in silico and glycomics microarray profiling approach. Integrative Biology, 11(7), 315-329. doi: 10.1093/intbio/zyz026en_IE
dc.identifier.issn1757-9708
dc.identifier.urihttp://hdl.handle.net/10379/15666
dc.description.abstractCommensal bacteria must colonize host mucosal surfaces to exert health-promoting properties, and bind to gastrointestinal tract (GIT) mucins via their cell surface adhesins. Considerable effort has been directed towards discovery of pathogen adhesins and their ligands to develop anti-infective strategies; however, little is known about the lectin-like adhesins and associated carbohydrate ligands in commensals. In this study, an in silico approach was used to detect surface exposed adhesins in the human commensal Lactobacillus paracasei subsp. paracasei, a promising probiotic commonly used in dairy product fermentation that presents anti-microbial activity. Of the 13 adhesin candidates, 3 sortase-dependent pili clusters were identified in this strain and expression of the adhesin candidate genes was confirmed in vitro. Mass spectrometry analysis confirmed the presence of surface adhesin elongation factor Tu and the chaperonin GroEL, but not pili expression. Whole cells were subsequently incubated on microarrays featuring a panel of GIT mucins from nine different mammalian species and two human-derived cell lines and a library of carbohydrate structures. Binding profiles were compared to those of two known pili-producing lactobacilli, L. johnsonii and L. rhamnosus and all Lactobacillus species displayed overlapping but distinct signatures, which may indicate different abilities for regiospecific GIT colonization. In addition, L. paracasei whole cells favoured binding to ¿-(2 ¿ 3)-linked sialic acid and ¿-(1 ¿ 2)-linked fucose-containing carbohydrate structures including blood groups A, B and O and Lewis antigens x, y and b. This study furthers our understanding of host-commensal cross-talk by identifying potential adhesins and specific GIT mucin and carbohydrate ligands and provides insight into the selection of colonization sites by commensals in the GIT.en_IE
dc.description.sponsorshipThis work was supported by Science Foundation Ireland Strategic Research Cluster programme in support of Alimentary Glycoscience Research Cluster (grant number 08/SRC/B1393). MK is grateful to the Royal Society of Chemistry Analytical Chemistry Trust Fund (ACTF) for the ACTF Fellowship Award 2018. The authors acknowledge the Centre for Microscopy & Imaging at the National University of Ireland, Galway (NUI, Galway) funded by NUI Galway and the Irish Government Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007-2013. The authors thank Prof. Maarten Van de Guchte and Mr Valentin Loux, Unité Mathématique, Informatique et Génome, INRA, Jouy en Josas, France for help with SurfG+ analysis, Prof. Y. C. Lee, Department of Biology, Johns Hopkins University, Baltimore, Maryland, U.S.A., for the kind gift of pigeon egg white and Prof. Stephen D. Carrington, Dr. Mary E. Gallagher, Dr. Colm Reid and Dr. Marguerite Clyne of University College Dublin for the kind gift of mucins.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherOxford University Press (OUP)en_IE
dc.relation.ispartofIntegrative Biology : Quantitative Biosciences From Nano To Macroen
dc.subjectcarbohydratesadhesinsen_IE
dc.subjectbacterialantigenscellen_IE
dc.subjectlineschen_IE
dc.subjectaperoninen_IE
dc.subjectbacterialen_IE
dc.subjectfimbrialen_IE
dc.subjectactobacillusen_IE
dc.subjectlectinen_IE
dc.subjectligandsen_IE
dc.subjectmammalsen_IE
dc.subjectmucinsen_IE
dc.subjectpeptideen_IE
dc.subjectelongationen_IE
dc.subjectfactoren_IE
dc.subjecttumassen_IE
dc.subjectspectrometryen_IE
dc.subjectbacteriaen_IE
dc.subjectpathogenicen_IE
dc.subjectorganismen_IE
dc.subjectgastrointestinalen_IE
dc.subjecttractmicrobialen_IE
dc.subjectcolonizationen_IE
dc.subjectbinding (molecular function)en_IE
dc.subjectlactobacillusen_IE
dc.subjectparacaseien_IE
dc.titleIdentification of putative adhesins and carbohydrate ligands of Lactobacillus paracasei using a combinatorial in silico and glycomics microarray profiling approachen_IE
dc.typeArticleen_IE
dc.date.updated2020-01-03T08:54:16Z
dc.identifier.doi10.1093/intbio/zyz026
dc.local.publishedsourcehttps://doi.org/10.1093/intbio/zyz026en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderRoyal Society of Chemistry Analytical Chemistry Trust Funden_IE
dc.description.embargo2020-11-11
dc.internal.rssid19058701
dc.local.contactMichelle Kilcoyne, Glycosciences, School Of Natural Sciences, Nui Galway. 5885 Email: michelle.kilcoyne@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Strategic Research Cluster/08/SRC/B1393/IE/SRC AGRC: Glycoscience Research Cluster/en_IE
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