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dc.contributor.authorUtratna, Marta
dc.contributor.authorAnnuk, Heidi
dc.contributor.authorGerlach, Jared Q.
dc.contributor.authorLee, Yuan C.
dc.contributor.authorKane, Marian
dc.contributor.authorKilcoyne, Michelle
dc.contributor.authorJoshi, Lokesh
dc.date.accessioned2020-01-02T12:25:21Z
dc.date.available2020-01-02T12:25:21Z
dc.date.issued2017-07-25
dc.identifier.citationUtratna, Marta, Annuk, Heidi, Gerlach, Jared Q., Lee, Yuan C., Kane, Marian, Kilcoyne, Michelle, & Joshi, Lokesh. (2017). Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray. Scientific Reports, 7(1), 6477. doi: 10.1038/s41598-017-06797-6en_IE
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10379/15665
dc.description.abstractThere is an urgent need for discovery of novel antimicrobials and carbohydrate-based anti-adhesive strategies are desirable as they may not promote resistance. Discovery of novel anti-adhesive molecules from natural product libraries will require the use of a high throughput screening platform. Avian egg white (EW) provides nutrition for the embryo and protects against infection, with glycosylation responsible for binding certain pathogens. In this study, a microarray platform of 78 species of avian EWs was developed and profiled for glycosylation using a lectin panel with a wide range of carbohydrate specificities. The dominating linkages of sialic acid in EWs were determined for the first time using the lectins MAA and SNA-I. EW glycosylation similarity among the different orders of birds did not strictly depend on phylogenetic relationship. The interactions of five strains of bacterial pathogens, including Escherichia coli, Staphylococcus aureus and Vibrio cholera, identified a number of EWs as potential anti-adhesives, with some as strain-or species-specific. Of the two bacterial toxins examined, shiga-like toxin 1 subunit B bound to ten EWs with similar glycosylation more intensely than pigeon EW. This study provides a unique platform for high throughput screening of natural products for specific glycosylation and pathogen interactions. This platform may provide a useful platform in the future for discovery of anti-adhesives targeted for strain and species specificity.en_IE
dc.description.sponsorshipThis work was funded by Science Foundation Ireland (SFI) grant numbers 11/TIDA/I2044 in support of a Technology Innovation Development Award, 08/SRC/B1393 in support of the Alimentary Glycoscience Research Cluster and 07/SK/B1250 for Stokes Professor for Glycosciences (LJ), and the European Union FP7 grant number 260600 in support of the GlycoHIT consortium. The authors thank Prof. James O’Gara and Ms Andrea Flannery for the gift of S. aureus strains and Dr. Aoife Boyd for V. parahaemolyticus. Y.C.L. thanks the late Dr. M. Laskowski, Jr. for generously providing the invaluable egg white collection, which he was gifted over the course of many years by various donors including zoos, research institutes and game preserves. Bird eggs should not be collected from the wild without ethical approval from the relevant national or international governing body.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherNature Researchen_IE
dc.relation.ispartofScientific Reportsen
dc.subjectOVOMUCOID 3RD DOMAINSen_IE
dc.subjectAMINO-ACID-SEQUENCESen_IE
dc.subjectEDIBLE BIRDS NESTen_IE
dc.subjectN-GLYCANSen_IE
dc.subjectCAMPYLOBACTER-JEJUNIen_IE
dc.subjectANTIADHESION THERAPYen_IE
dc.subjectMAJOR GLYCOPROTEINSen_IE
dc.subjectHEN OVOMUCINen_IE
dc.subjectHUMAN HEALTHen_IE
dc.subjectEXPRESSIONen_IE
dc.titleRapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarrayen_IE
dc.typeArticleen_IE
dc.date.updated2020-01-02T12:10:36Z
dc.identifier.doi10.1038/s41598-017-06797-6
dc.local.publishedsourcehttps://doi.org/10.1038/s41598-017-06797-6
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderSeventh Framework Programmeen_IE
dc.internal.rssid13001917
dc.local.contactMichelle Kilcoyne, Glycosciences, School Of Natural Sciences, Nui Galway. 5885 Email: michelle.kilcoyne@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/11/TIDA/I2044/IE/Glycoprotein micelle mediated capture of bacteria/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Strategic Research Cluster/08/SRC/B1393/IE/SRC AGRC: Glycoscience Research Cluster/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::HEALTH/260600/EU/Glycomics by High-throughput Integrated Technologies/GLYCOHITen_IE
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