Show simple item record

dc.contributor.authorBahri, Ola A.
dc.contributor.authorNaldaiz-Gastesi, Neia
dc.contributor.authorKennedy, Donna C.
dc.contributor.authorWheatley, Antony M.
dc.contributor.authorIzeta, Ander
dc.contributor.authorMcCullagh, Karl J. A.
dc.date.accessioned2019-11-08T08:11:43Z
dc.date.available2019-11-08T08:11:43Z
dc.date.issued2019-11-04
dc.identifier.citationBahri, Ola A., Naldaiz-Gastesi, Neia, Kennedy, Donna C., Wheatley, Antony M., Izeta, Ander, & McCullagh, Karl J. A. (2019). The panniculus carnosus muscle: A novel model of striated muscle regeneration that exhibits sex differences in the mdx mouse. Scientific Reports, 9(1), 15964. doi: 10.1038/s41598-019-52071-2en_IE
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10379/15553
dc.description.abstractThe dermal striated muscle panniculus carnosus (PC), prevalent in lower mammals with remnants in humans, is highly regenerative, and whose function is purported to be linked to defence and shivering thermogenesis. Given the heterogeneity of responses of different muscles to disease, we set out to characterize the PC in wild-type and muscular dystrophic mdx mice. The mouse PC contained mainly fast-twitch type IIB myofibers showing body wide distribution. The PC exemplified heterogeneity in myofiber sizes and a prevalence of central nucleated fibres (CNFs), hallmarks of regeneration, in wild-type and mdx muscles, which increased with age. PC myofibers were hypertrophic in mdx compared to wild-type mice. Sexual dimorphism was apparent with a two-fold increase in CNFs in PC from male versus female mdx mice. To evaluate myogenic potential, PC muscle progenitors were isolated from 8-week old wild-type and mdx mice, grown and differentiated for 7-days. Myogenic profiling of PC-derived myocytes suggested that male mdx satellite cells (SCs) were more myogenic than female counterparts, independent of SC density in PC muscles. Muscle regenerative differences in the PC were associated with alterations in expression of calcium handling regulatory proteins. These studies highlight unique aspects of the PC muscle and its potential as a model to study mechanisms of striated muscle regeneration in health and disease.en_IE
dc.description.sponsorshipWe thank Barbara Coen in Physiology, NUI Galway for initial guidance in preparing mouse dorsal skin tissue. The authors acknowledge the facilities and technical assistance of the NCBES qPCR Facility, funded by National University of Ireland Galway and the Irish Government’s Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007–2013. We thank Dr. Francina Munell at Vall d’Hebron Institut de Recerca for help with early preliminary PC cell culture experiments. This work was supported by funding from the Ministry of higher education, Saudi Arabia to O.B. and K. M. C. at NUI Galway and funding to K. M. C. from the Millennium Research fund, NUI Galway. N.N.-G. received a studentship from the Department of Education, University and Research of the Basque Government (PRE2013-1-1168). D.K. received an Irish Research Council Government of Ireland Postgraduate Scholarship (GOIPG/2018/2622) and College of Medicine, NUI Galway, PhD Support Fund. A.I. was funded by ISCIII (PI16/01430), co-funded by ERDF/ESF, “Investing in your future”, and the Basque Government (KK-2019/00093).en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherNature Researchen_IE
dc.relation.ispartofScientific Reportsen
dc.subjectMuscle stem cellsen_IE
dc.subjectNeuromuscular diseaseen_IE
dc.subjectSkeletal muscleen_IE
dc.titleThe panniculus carnosus muscle: A novel model of striated muscle regeneration that exhibits sex differences in the mdx mouse.en_IE
dc.typeArticleen_IE
dc.date.updated2019-11-08T07:58:16Z
dc.identifier.doi10.1038/s41598-019-52071-2
dc.local.publishedsourcehttps://doi.org/10.1038/s41598-019-52071-2en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderMinistry of Higher Education, Saudi Arabiaen_IE
dc.contributor.funderMillennium Research Fund, NUI Galwayen_IE
dc.contributor.funderDepartment of Education, University and Research of the Basque Governmenten_IE
dc.contributor.funderIrish Research Councilen_IE
dc.contributor.funderCollege of Medicine, Nursing and Health Sciences, NUI Galwayen_IE
dc.contributor.funderEuropean Regional Development Funden_IE
dc.internal.rssid18356273
dc.local.contactKarl Mccullagh, Dept Of Physiology, Quadrangle Building, Nui Galway. 4220 Email: karl.mccullagh@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
nui.item.downloads13


Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:

Thumbnail

This item appears in the following Collection(s)

Show simple item record