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dc.contributor.authorZhang, Xiao-Xuan
dc.contributor.authorCwiklinski, Krystyna
dc.contributor.authorHu, Rui-Si
dc.contributor.authorZheng, Wen-Bin
dc.contributor.authorSheng, Zhao-An
dc.contributor.authorZhang, Fu-Kai
dc.contributor.authorElsheikha, Hany M.
dc.contributor.authorDalton, John P.
dc.contributor.authorZhu, Xing-Quan
dc.date.accessioned2019-10-15T10:15:36Z
dc.date.available2019-10-15T10:15:36Z
dc.date.issued2019-10-12
dc.identifier.citationZhang, Xiao-Xuan, Cwiklinski, Krystyna, Hu, Rui-Si, Zheng, Wen-Bin, Sheng, Zhao-An, Zhang, Fu-Kai, Elsheikha, Hany M., Dalton, John P., Zhu, Xing-Quan. (2019). Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts. BMC Genomics, 20(1), 729. doi: 10.1186/s12864-019-6103-5en_IE
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/10379/15517
dc.description.abstractThe tropical liver fluke, Fasciola gigantica causes fasciolosis, an important disease of humans and livestock. We characterized dynamic transcriptional changes associated with the development of the parasite in its two hosts, the snail intermediate host and the mammalian definitive host. Differential gene transcription analysis revealed 7445 unigenes transcribed by all F. gigantica lifecycle stages, while the majority (n¿=¿50,977) exhibited stage-specific expression. Miracidia that hatch from eggs are highly transcriptionally active, expressing a myriad of genes involved in pheromone activity and metallopeptidase activity, consistent with snail host finding and invasion. Clonal expansion of rediae within the snail correlates with increased expression of genes associated with transcription, translation and repair. All intra-snail stages (miracidia, rediae and cercariae) require abundant cathepsin L peptidases for migration and feeding and, as indicated by their annotation, express genes putatively involved in the manipulation of snail innate immune responses. Cercariae emerge from the snail, settle on vegetation and become encysted metacercariae that are infectious to mammals; these remain metabolically active, transcribing genes involved in regulation of metabolism, synthesis of nucleotides, pH and endopeptidase activity to assure their longevity and survival on pasture. Dramatic growth and development following infection of the mammalian host are associated with high gene transcription of cell motility pathways, and transport and catabolism pathways. The intra-mammalian stages temporally regulate key families of genes including the cathepsin L and B proteases and their trans-activating peptidases, the legumains, during intense feeding and migration through the intestine, liver and bile ducts. While 70% of the F. gigantica transcripts share homology with genes expressed by the temperate liver fluke Fasciola hepatica, gene expression profiles of the most abundantly expressed transcripts within the comparable lifecycle stages implies significant species-specific gene regulation. Transcriptional profiling of the F. gigantica lifecycle identified key metabolic, growth and developmental processes the parasite undergoes as it encounters vastly different environments within two very different hosts. Comparative analysis with F. hepatica provides insight into the similarities and differences of these parasites that diverged >¿20 million years ago, crucial for the future development of novel control strategies against both species.en_IE
dc.description.sponsorshipThis work was supported by the National Key Basic Research Program (973 Program) of China (Grant No. 2015CB150300) and the Elite Program of Chinese Academy of Agricultural Sciences. JPD is a recipient of a Science Foundation Ireland (SFI, Republic of Ireland) Professorship in Molecular Parasitology.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherBMC (part of Springer Nature)en_IE
dc.relation.ispartofBMC Genomicsen
dc.subjectFasciola giganticaen_IE
dc.subjectDifferent developmental stagesen_IE
dc.subjectHost-pathogen interactionen_IE
dc.subjectRNA-sequencingen_IE
dc.subjectDe novo Transcriptomeen_IE
dc.titleComplex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts.en_IE
dc.typeArticleen_IE
dc.date.updated2019-10-14T13:27:22Z
dc.identifier.doi10.1186/s12864-019-6103-5
dc.local.publishedsourcehttps://doi.org/10.1186/s12864-019-6103-5en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderNational Basic Research Program of China (973 Program)en_IE
dc.contributor.funderElite Program of Chinese Academy of Agricultural Sciencesen_IE
dc.internal.rssid18037931
dc.local.contactKrystyna Cwiklinski. Email: krystyna.cwiklinski@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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