Hypoxia activates the PTHrP-MEF2C pathway to attenuate hypertrophy in mesenchymal stem cell derived cartilage
dc.contributor.author | Browe, David C. | |
dc.contributor.author | Coleman, Cynthia M. | |
dc.contributor.author | Barry, Frank P. | |
dc.contributor.author | Elliman, Stephen J. | |
dc.date.accessioned | 2019-10-11T08:58:03Z | |
dc.date.available | 2019-10-11T08:58:03Z | |
dc.date.issued | 2019-09-16 | |
dc.identifier.citation | Browe, David C., Coleman, Cynthia M., Barry, Frank P., & Elliman, Stephen J. (2019). Hypoxia Activates the PTHrP –MEF2C Pathway to Attenuate Hypertrophy in Mesenchymal Stem Cell Derived Cartilage. Scientific Reports, 9(1), 13274. doi: 10.1038/s41598-019-49499-x | en_IE |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10379/15507 | |
dc.description.abstract | Articular cartilage lacks an intrinsic repair capacity and due to the ability of mesenchymal stem cells (MSCs) to differentiate into chondrocytes, MSCs have been touted as a cellular source to regenerate damaged cartilage. However, a number of prevailing concerns for such a treatment remain. Generally, administration of MSCs into a cartilage defect results in poor regeneration of the damaged cartilage with the repaired cartilage consisting primarily of fibro-cartilage rather than hyaline cartilage. Methods that improve the chondrogenic potential of transplanted MSCs in vivo may be advantageous. In addition, the proclivity of MSC-derived cartilage to undergo hypertrophic differentiation or form bone in vivo also remains a clinical concern. If MSC-derived cartilage was to undergo hypertrophic differentiation in vivo, this would be deleterious in a clinical setting. This study focuses on establishing a mechanism of action by which hypoxia or low oxygen tension can be used to both enhance chondrogenesis and attenuate hypertrophic differentiation of both MSC and ATDC5 derived chondrocytes. Having elucidated a novel mechanism of action, the subsequent goals of this study were to develop an in vitro culture regime to mimic the beneficial effects of physiological low oxygen tension in a normoxic environment. | en_IE |
dc.description.sponsorship | Science Foundation Ireland (SFI 09/SRC/B1794) and FP7 Health (223298). | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | Nature Research | en_IE |
dc.relation.ispartof | Scientific Reports | en |
dc.subject | IN-VITRO CHONDROGENESIS | en_IE |
dc.subject | HORMONE-RELATED PROTEIN | en_IE |
dc.subject | LINE | en_IE |
dc.subject | DIFFERENTIATION | en_IE |
dc.subject | CALCIFICATION | en_IE |
dc.subject | PROMOTES | en_IE |
dc.subject | ATDC5 | en_IE |
dc.subject | TRANSPLANTATION | en_IE |
dc.subject | CHONDROCYTES | en_IE |
dc.subject | EXPRESSION | en_IE |
dc.title | Hypoxia activates the PTHrP-MEF2C pathway to attenuate hypertrophy in mesenchymal stem cell derived cartilage | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2019-10-11T08:48:57Z | |
dc.identifier.doi | 10.1038/s41598-019-49499-x | |
dc.local.publishedsource | https://doi.org/10.1038/s41598-019-49499-x | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | Science Foundation Ireland | en_IE |
dc.contributor.funder | Seventh Framework Programme | en_IE |
dc.internal.rssid | 17837364 | |
dc.local.contact | Cynthia Coleman, School Of Medicine, Nui Galway. 5852 Email: cynthia.coleman@nuigalway.ie | |
dc.local.copyrightchecked | Yes | |
dc.local.version | ACCEPTED | |
dcterms.project | info:eu-repo/grantAgreement/SFI/SFI Strategic Research Cluster/09/SRC/B1794/IE/SRC REMEDI: REMEDI-2: From Research to Human Therapy/ | en_IE |
dcterms.project | info:eu-repo/grantAgreement/EC/FP7::SP1::HEALTH/223298/EU/Utilisation of the mesenchymal stem cell receptome for rational development of uniform, serum-free culture conditions and tools for cell characterization/PURSTEM | en_IE |
nui.item.downloads | 33 |
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