Show simple item record

dc.contributor.authorBrowe, David C.
dc.contributor.authorColeman, Cynthia M.
dc.contributor.authorBarry, Frank P.
dc.contributor.authorElliman, Stephen J.
dc.date.accessioned2019-10-11T08:58:03Z
dc.date.available2019-10-11T08:58:03Z
dc.date.issued2019-09-16
dc.identifier.citationBrowe, David C., Coleman, Cynthia M., Barry, Frank P., & Elliman, Stephen J. (2019). Hypoxia Activates the PTHrP –MEF2C Pathway to Attenuate Hypertrophy in Mesenchymal Stem Cell Derived Cartilage. Scientific Reports, 9(1), 13274. doi: 10.1038/s41598-019-49499-xen_IE
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10379/15507
dc.description.abstractArticular cartilage lacks an intrinsic repair capacity and due to the ability of mesenchymal stem cells (MSCs) to differentiate into chondrocytes, MSCs have been touted as a cellular source to regenerate damaged cartilage. However, a number of prevailing concerns for such a treatment remain. Generally, administration of MSCs into a cartilage defect results in poor regeneration of the damaged cartilage with the repaired cartilage consisting primarily of fibro-cartilage rather than hyaline cartilage. Methods that improve the chondrogenic potential of transplanted MSCs in vivo may be advantageous. In addition, the proclivity of MSC-derived cartilage to undergo hypertrophic differentiation or form bone in vivo also remains a clinical concern. If MSC-derived cartilage was to undergo hypertrophic differentiation in vivo, this would be deleterious in a clinical setting. This study focuses on establishing a mechanism of action by which hypoxia or low oxygen tension can be used to both enhance chondrogenesis and attenuate hypertrophic differentiation of both MSC and ATDC5 derived chondrocytes. Having elucidated a novel mechanism of action, the subsequent goals of this study were to develop an in vitro culture regime to mimic the beneficial effects of physiological low oxygen tension in a normoxic environment.en_IE
dc.description.sponsorshipScience Foundation Ireland (SFI 09/SRC/B1794) and FP7 Health (223298).en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherNature Researchen_IE
dc.relation.ispartofScientific Reportsen
dc.subjectIN-VITRO CHONDROGENESISen_IE
dc.subjectHORMONE-RELATED PROTEINen_IE
dc.subjectLINEen_IE
dc.subjectDIFFERENTIATIONen_IE
dc.subjectCALCIFICATIONen_IE
dc.subjectPROMOTESen_IE
dc.subjectATDC5en_IE
dc.subjectTRANSPLANTATIONen_IE
dc.subjectCHONDROCYTESen_IE
dc.subjectEXPRESSIONen_IE
dc.titleHypoxia activates the PTHrP-MEF2C pathway to attenuate hypertrophy in mesenchymal stem cell derived cartilageen_IE
dc.typeArticleen_IE
dc.date.updated2019-10-11T08:48:57Z
dc.identifier.doi10.1038/s41598-019-49499-x
dc.local.publishedsourcehttps://doi.org/10.1038/s41598-019-49499-xen_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderSeventh Framework Programmeen_IE
dc.internal.rssid17837364
dc.local.contactCynthia Coleman, School Of Medicine, Nui Galway. 5852 Email: cynthia.coleman@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Strategic Research Cluster/09/SRC/B1794/IE/SRC REMEDI: REMEDI-2: From Research to Human Therapy/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::HEALTH/223298/EU/Utilisation of the mesenchymal stem cell receptome for rational development of uniform, serum-free culture conditions and tools for cell characterization/PURSTEMen_IE
nui.item.downloads33


Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:

Thumbnail

This item appears in the following Collection(s)

Show simple item record