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dc.contributor.authorSiggins, Alma
dc.contributor.authorEnright, Anne-Marie
dc.contributor.authorAbram, Florence
dc.contributor.authorBotting, Catherine
dc.contributor.authorO'Flaherty, Vincent
dc.date.accessioned2019-09-11T14:22:59Z
dc.date.available2019-09-11T14:22:59Z
dc.date.issued2012-09-14
dc.identifier.citationSiggins, Alma, Enright, Anne-Marie, Abram, Florence, Botting, Catherine, & O'Flaherty, Vincent. (2012). Impact of Trichloroethylene Exposure on the Microbial Diversity and Protein Expression in Anaerobic Granular Biomass at 37°C and 15°C. Archaea, 2012, 13. doi: 10.1155/2012/940159en_IE
dc.identifier.issn1472-3646
dc.identifier.urihttp://hdl.handle.net/10379/15426
dc.description.abstractGranular biomass from a laboratory-scale anaerobic bioreactor trial was analysed to identify changes in microbial community structure and function in response to temperature and trichloroethylene (TCE). Two bioreactors were operated at 37 degrees C, while two were operated at 15 degrees C. At the time of sampling, one of each temperature pair of bioreactors was exposed to process failure-inducing concentrations of TCE (60 mg L-1) while the other served as a TCE-free control. Bacterial community structure was investigated using denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone library analysis. Temperature was identified as an important factor for bacterial community composition, while minor differences were associated with trichloroethylene supplementation. Proteobacteria was the dominant phylum in all bioreactors, while clone library analysis revealed a higher proportion of Bacteroidetes-, Chloroflexi-, and Firmicutes-like clones at 15 degrees C than at 37 degrees C. Comparative metaproteomics in the presence and absence of TCE was carried out by two-dimensional gel electrophoresis (2-DGE), and 28 protein spots were identified, with putative functions related to cellular processes, including methanogenesis, glycolysis, the glyoxylate cycle, and the methyl malonyl pathway. A good agreement between metaproteomic species assignment and phylogenetic information was observed, with 10 of the identified proteins associated with members of the phylum Proteobacteria.en_IE
dc.description.sponsorshipThis work was supported by Enterprise Ireland, The Irish Environmental Protection Agency, and Science Foundation Ireland.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherHindawi Publishing Corporationen_IE
dc.relation.ispartofArchaea-An International Microbiological Journalen
dc.subjectWASTE-WATER TREATMENTen_IE
dc.subject16S RIBOSOMAL-RNAen_IE
dc.subjectDEGREES-Cen_IE
dc.subjectMETHANOGENIC ACTIVITYen_IE
dc.subjectSLUDGEen_IE
dc.subjectCOMMUNITYen_IE
dc.subjectDIGESTIONen_IE
dc.subjectPROPIONATEen_IE
dc.subjectBACTERIUMen_IE
dc.subjectARCHAEAen_IE
dc.titleImpact of trichloroethylene exposure on the microbial diversity and protein expression in anaerobic granular biomass at 37°C and 15°Cen_IE
dc.typeArticleen_IE
dc.date.updated2019-08-08T08:52:59Z
dc.identifier.doi10.1155/2012/940159
dc.local.publishedsourcehttp://dx.doi.org/10.1155/2012/940159en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderEnterprise Irelanden_IE
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderEnvironmental Protection Agency, Irelanden_IE
dc.internal.rssid16224660
dc.local.contactVincent O'Flaherty, Dept. Of Microbiology & Eci, Arts/Science Building, Nui Galway. 3734 Email: vincent.oflaherty@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
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