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dc.contributor.authorMadden, Pádhraig
dc.contributor.authorAl-Raei, Abdul M.
dc.contributor.authorEnright, Anne M.
dc.contributor.authorChinalia, Fabio A.
dc.contributor.authorde Beer, Dirk
dc.contributor.authorO'Flaherty, Vincent
dc.contributor.authorCollins, Gavin
dc.date.accessioned2019-09-11T13:42:42Z
dc.date.available2019-09-11T13:42:42Z
dc.date.issued2014-07-24
dc.identifier.citationMadden, Pádhraig, Al-Raei, Abdul M., Enright, Anne M., Chinalia, Fabio A., de Beer, Dirk, O'Flaherty, Vincent, & Collins, Gavin. (2014). Effect of sulfate on low-temperature anaerobic digestion. Frontiers in Microbiology, 5(376). doi: 10.3389/fmicb.2014.00376en_IE
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10379/15424
dc.description.abstractThe effect of sulfate addition on the stability of, and microbial community behavior in, low-temperature anaerobic expanded granular sludge bed-based bioreactors was investigated at 15 degrees C. Efficient bioreactor performance was observed, with chemical oxygen demand (COD) removal efficiencies of >90%, and a mean SO42- removal rate of 98.3%. In situ methanogensis appeared unaffected at a COD: SO42- influent ratio of 8:1, and subsequently of 3:1, and was impacted marginally only when the COD: SO42- ratio was 1:2. Specific methanogenic activity assays indicated a complex set of interactions between sulfate-reducing bacteria (SRB), methanogens and homoacetogenic bacteria. SO42- addition resulted in predominantly acetoclastic, rather than hydrogenotrophic, methanogenesis until >600 days of SO42--influenced bioreactor operation. Temporal microbial community development was monitored by denaturation gradient gel electrophoresis (DGGE) of 16S rRNA genes. Fluorescence in situ hybridizations (FISH), qPCR and microsensor analysis were combined to investigate the distribution of microbial groups, and particularly SRB and methanogens, along the structure of granular biofilms. qPCR data indicated that sulfidogenic genes were present in methanogenic and sulfidogenic biofilms, indicating the potential for sulfate reduction even in bioreactors not exposed to SO42-. Although the architecture of methanogenic and sulfidogenic granules was similar, indicating the presence of SRB even in methanogenic systems, FISH with rRNA targets found that the SRB were more abundant in the sulfidogenic biofilms. Methanosaeta species were the predominant, keystone members of the archaeal community, with the complete absence of the Methanosarcina species in the experimental bioreactor by trial conclusion. Microsensor data suggested the ordered distribution of sulfate reduction and sulfide accumulation, even in methanogenic granules.en_IE
dc.description.sponsorshipPádhraig Madden was supported by a scholarship from the Irish Research Council. Gavin Collins is supported by a European Research Council (ERC) Starting Grant (‘3C-BIOTECH’; project no. 261330). Profs. Michael Böttcher and Tim Ferdelman, and Dr. Raeid Abed, are thanked for their insightful conversations.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherFrontiers Mediaen_IE
dc.relation.ispartofFrontiers In Microbiologyen
dc.subjectbiogasen_IE
dc.subjectlow-temperature anaerobic digestionen_IE
dc.subjectsulfateen_IE
dc.subjectsulfideen_IE
dc.subjectmethaneen_IE
dc.subjectmethanogenesisen_IE
dc.subjectwastewateren_IE
dc.subjectWASTE-WATER TREATMENTen_IE
dc.subjectVOLATILE FATTY-ACIDen_IE
dc.subject16S RIBOSOMAL-RNAen_IE
dc.subjectTARGETED OLIGONUCLEOTIDE PROBESen_IE
dc.subjectMICROBIAL COMMUNITY STRUCTUREen_IE
dc.subjectPOLYMERASE-CHAIN-REACTIONen_IE
dc.subjectIN-SITU HYBRIDIZATIONen_IE
dc.subjectREDUCING BACTERIAen_IE
dc.subjectMETHANOGENIC ACTIVITYen_IE
dc.subjectBIOLOGICAL TREATMENTen_IE
dc.titleEffect of sulfate on low-temperature anaerobic digestionen_IE
dc.typeArticleen_IE
dc.date.updated2019-08-02T10:59:57Z
dc.identifier.doi10.3389/fmicb.2014.00376
dc.local.publishedsourcehttps://doi.org/10.3389/fmicb.2014.00376en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderIrish Research Councilen_IE
dc.contributor.funderEuropean Research Councilen_IE
dc.internal.rssid6971433
dc.local.contactVincent O'Flaherty, Dept. Of Microbiology & Eci, Arts/Science Building, Nui Galway. 3734 Email: vincent.oflaherty@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
dcterms.projectinfo:eu-repo/grantAgreement/EC/FP7::SP2::ERC/261330/EU/Cold Carbon Catabolism of Microbial Communities underprinning a Sustainable Bioenergy and Biorefinery Economy/3CBIOTECHen_IE
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