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dc.contributor.authorGunnigle, Eoin
dc.contributor.authorNielsen, Jeppe L.
dc.contributor.authorFuszard, Matthew
dc.contributor.authorBotting, Catherine H.
dc.contributor.authorSheahan, Jerome
dc.contributor.authorO'Flaherty, Vincent
dc.contributor.authorAbram, Florence
dc.date.accessioned2019-09-11T13:22:13Z
dc.date.available2019-09-11T13:22:13Z
dc.date.issued2015-10-27
dc.identifier.citationGunnigle, Eoin, Nielsen, Jeppe L., Fuszard, Matthew, Botting, Catherine H., Sheahan, Jerome, O'Flaherty, Vincent, & Abram, Florence. (2015). Functional responses and adaptation of mesophilic microbial communities to psychrophilic anaerobic digestion. FEMS Microbiology Ecology, 91(12). doi: 10.1093/femsec/fiv132en_IE
dc.identifier.issn1574-6941
dc.identifier.urihttp://hdl.handle.net/10379/15423
dc.description.abstractPsychrophilic (<20°C) anaerobic digestion (AD) represents an attractive alternative to mesophilic wastewater treatment. In order to investigate the AD microbiome response to temperature change, with particular emphasis on methanogenic archaea, duplicate laboratory-scale AD bioreactors were operated at 37°C followed by a temperature drop to 15°C. A volatile fatty acid-based wastewater (composed of propionic acid, butyric acid, acetic acid and ethanol) was used to provide substrates representing the later stages of AD. Community structure was monitored using 16S rRNA gene clone libraries, as well as DNA and cDNA-based DGGE analysis, while the abundance of relevant methanogens was followed using qPCR. In addition, metaproteomics, microautoradiography–fluorescence in situ hybridization, and methanogenic activity measurements were employed to investigate microbial activities and functions. Methanomicrobiales abundance increased at low temperature, which correlated with an increased contribution of CH4 production from hydrogenotrophic methanogenesis at 15°C. Methanosarcinales utilized acetate and H2/CO2 as CH4 precursors at both temperatures and a partial shift from acetoclastic to hydrogenotrophic methanogenesis was observed for this archaeal population at 15°C. An upregulation of protein expression was reported at low temperature as well as the detection of chaperones indicating that mesophilic communities experienced stress during long-term exposure to 15°C. Overall, changes in microbial community structure and function were found to underpin the adaptation of mesophilic sludge to psychrophilic AD.en_IE
dc.description.sponsorshipThis work was supported by Science Foundation Ireland (Grants RFP 08/RFP/EOB1343 and 06/CP/E006) and the Wellcome Trust (grant number GR06281AIA), which funded the purchase of the QStar XL mass spectrometer at the BSRC Mass Spectrometry and Proteomics Facility, University of St Andrews.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherOxford University Pressen_IE
dc.relation.ispartofFems Microbiology Ecologyen
dc.subjectanaerobic digestionen_IE
dc.subjectmetaproteomicsen_IE
dc.subjectmicrobial phylogenetic diversityen_IE
dc.subjectmethanogenesisen_IE
dc.subjectpsychrophilicen_IE
dc.subjectMAR-FISHen_IE
dc.titleFunctional responses and adaptation of mesophilic microbial communities to psychrophilic anaerobic digestionen_IE
dc.typeArticleen_IE
dc.date.updated2019-08-02T10:44:47Z
dc.identifier.doi10.1093/femsec/fiv132
dc.local.publishedsourcehttps://doi.org/10.1093/femsec/fiv132en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderWellcome Trusten_IE
dc.internal.rssid10170642
dc.local.contactVincent O'Flaherty, Dept. Of Microbiology & Eci, Arts/Science Building, Nui Galway. 3734 Email: vincent.oflaherty@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Frontiers Programme (RFP)/08/RFP/EOB1343/IE/Towards an understanding of cold adaptation in methanogenic consortia: the application of metaproteomic analyses to anaerobic digestion/en_IE
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