Show simple item record

dc.contributor.authorKeating, Ciara
dc.contributor.authorChin, Jason P.
dc.contributor.authorHughes, Dermot
dc.contributor.authorManesiotis, Panagiotis
dc.contributor.authorCysneiros, Denise
dc.contributor.authorMahony, Therese
dc.contributor.authorSmith, Cindy J.
dc.contributor.authorMcGrath, John W.
dc.contributor.authorO'Flaherty, Vincent
dc.identifier.citationKeating, Ciara, Chin, Jason P., Hughes, Dermot, Manesiotis, Panagiotis, Cysneiros, Denise, Mahony, Therese, Smith,Cindy J., McGrath,John W., O’Flaherty, Vincent. (2016). Biological Phosphorus Removal During High-Rate, Low-Temperature, Anaerobic Digestion of Wastewater. Frontiers in Microbiology, 7(226). doi: 10.3389/fmicb.2016.00226en_IE
dc.description.abstractWe report, for the first time, extensive biologically mediated phosphate removal from wastewater during high-rate anaerobic digestion (AD). A hybrid sludge bed/fixed-film (packed pumice stone) reactor was employed for low-temperature (12°C) anaerobic treatment of synthetic sewage wastewater. Successful phosphate removal from the wastewater (up to 78% of influent phosphate) was observed, mediated by biofilms in the reactor. Scanning electron microscopy and energy dispersive X-ray analysis revealed the accumulation of elemental phosphorus (~2%) within the sludge bed and fixed-film biofilms. 4', 6-diamidino-2-phenylindole (DAPI) staining indicated phosphorus accumulation was biological in nature and mediated through the formation of intracellular inorganic polyphosphate (polyP) granules within these biofilms. DAPI staining further indicated that polyP accumulation was rarely associated with free cells. Efficient and consistent chemical oxygen demand (COD) removal was recorded, throughout the 732-day trial, at applied organic loading rates between 0.4 and 1.5 kg COD m(-3) d(-1) and hydraulic retention times of 8-24 h, while phosphate removal efficiency ranged from 28 to 78% on average per phase. Analysis of protein hydrolysis kinetics and the methanogenic activity profiles of the biomass revealed the development, at 12°C, of active hydrolytic and methanogenic populations. Temporal microbial changes were monitored using Illumina MiSeq analysis of bacterial and archaeal 16S rRNA gene sequences. The dominant bacterial phyla present in the biomass at the conclusion of the trial were the Proteobacteria and Firmicutes and the dominant archaeal genus was Methanosaeta. Trichococcus and Flavobacterium populations, previously associated with low temperature protein degradation, developed in the reactor biomass. The presence of previously characterized polyphosphate accumulating organisms (PAOs) such as Rhodocyclus, Chromatiales, Actinobacter, and Acinetobacter was recorded at low numbers. However, it is unknown as yet if these were responsible for the luxury polyP uptake observed in this system. The possibility of efficient phosphate removal and recovery from wastewater during AD would represent a major advance in the scope for widespread application of anaerobic wastewater treatment technologies.en_IE
dc.description.sponsorshipThis work was supported by the Science Foundation Ireland Charles Parsons Award (06_CP_E006), Investigator Programme Grant (14/IA/2371), Science Foundation Ireland (14/IA/2371), and the Irish Environmental Protection Agency (2014-W-LS-7). CJS is supported by Science Foundation Ireland Starting Investigator-COFUND fellowship (11/SIRG/B2159).en_IE
dc.publisherFrontiers Mediaen_IE
dc.relation.ispartofFrontiers In Microbiologyen
dc.subjectBiological Phosphorus Removalen_IE
dc.subjectAnaerobic Digestionen_IE
dc.titleBiological phosphorus removal during high-rate, low-temperature, anaerobic digestion of wastewateren_IE
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderEnvironmental Protection Agency, Irelanden_IE
dc.local.contactVincent O'Flaherty, Dept. Of Microbiology & Eci, Arts/Science Building, Nui Galway. 3734 Email:
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/14/IA/2371/IE/i_PAD: Innovative biological phosphate (bioP) and anaerobic digestion (AD) technology for waste treatment, energy generation and phosphorus recovery./en_IE
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/11/SIRG/B2159/IE/Molecular Microbial Ecology of Ammonia Oxidation in Coastal Bay Sediments/en_IE

Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:


This item appears in the following Collection(s)

Show simple item record