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dc.contributor.authorTonoyan, Lilit
dc.contributor.authorFleming, Gerard T. A.
dc.contributor.authorMc Cay, Paul H.
dc.contributor.authorFriel, Ruairi
dc.contributor.authorO'Flaherty, Vincent
dc.date.accessioned2019-09-11T12:50:40Z
dc.date.available2019-09-11T12:50:40Z
dc.date.issued2017-05-02
dc.identifier.citationTonoyan, Lilit, Fleming, Gerard T. A., Mc Cay, Paul H., Friel, Ruairi, & O'Flaherty, Vincent. (2017). Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems. Frontiers in Microbiology, 8(680). doi: 10.3389/fmicb.2017.00680en_IE
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10379/15421
dc.description.abstractAntibiotic resistance is an increasingly serious threat to global health. Consequently, the development of non-antibiotic based therapies and disinfectants, which avoid induction of resistance, or cross-resistance, is of high priority. We report the synthesis of a biocidal complex, which is produced by the reaction between ionic oxidizable saltsiodide and thiocyanatein the presence of hydrogen peroxide as an oxidation source. The reaction generates bactericidal reactive oxygen and iodine species. In this study, we report that the iodo-thiocyanate complex (ITC) is an effective bactericidal agent with activity against planktonic and biofilm cells of Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and methicillinresistant S. aureus) bacteria. The minimum bactericidal concentrations and the minimum biofilm eradication concentrations of the biocidal composite were in the range of 7.831.3 and 31.3-250 mu g ml(-1) , respectively. As a result, the complex was capable to cause a rapid cell death of planktonic test cultures at between 0.5 and 2 h, and complete eradication of dual and mono-species biofilms between 30 s and 10 min. Furthermore, the test bacteria, including a MRSA strain, exposed to the cocktail failed to develop resistance after serial passages. The antimicrobial activity of the ITC appears to derive from the combinational effect of the powerful species capable of oxidizing the essential biomolecules of bacteria. The use of this composition may provide an effective and efficient method for killing potential pathogens, as well as for disinfecting and removing biofilm contamination.en_IE
dc.description.sponsorshipThis work was funded by the Irish Research Council Government of Ireland Postgraduate Scholarship Scheme (GOIPG/2013/584) and by Enterprise Ireland (CF/2011/1605).en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherFrontiers Mediaen_IE
dc.relation.ispartofFrontiers In Microbiologyen
dc.subjectiodo-thiocyanate complex (ITC)en_IE
dc.subjectbiocideen_IE
dc.subjectiodineen_IE
dc.subjectin vitro susceptibilityen_IE
dc.subjectbactericidalen_IE
dc.subjectLACTOPEROXIDASE SYSTEMen_IE
dc.subjectSTAPHYLOCOCCUS-AUREUSen_IE
dc.subjectESCHERICHIA-COLIen_IE
dc.subjectOXIDATIVE STRESSen_IE
dc.subjectBIOFILMSen_IE
dc.subjectSUSCEPTIBILITYen_IE
dc.subjectIODINEen_IE
dc.subjectTHIOCYANATEen_IE
dc.subjectMECHANISMSen_IE
dc.subjectVIABILITYen_IE
dc.titleAntibacterial potential of an antimicrobial agent inspired by peroxidase-catalyzed systemsen_IE
dc.typeArticleen_IE
dc.date.updated2019-08-02T09:49:02Z
dc.identifier.doi10.3389/fmicb.2017.00680
dc.local.publishedsourcehttps://doi.org/10.3389/fmicb.2017.00680en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderIrish Research Councilen_IE
dc.contributor.funderEnterprise Irelanden_IE
dc.internal.rssid12654740
dc.local.contactVincent O'Flaherty, Dept. Of Microbiology & Eci, Arts/Science Building, Nui Galway. 3734 Email: vincent.oflaherty@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
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