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dc.contributor.authorTonoyan, Lilit
dc.contributor.authorBoyd, Aoife
dc.contributor.authorFleming, Gerard T.A.
dc.contributor.authorFriel, Ruairi
dc.contributor.authorGately, Carol M.
dc.contributor.authorMc Cay, Paul H.
dc.contributor.authorO'Flaherty, Vincent
dc.date.accessioned2019-08-29T13:58:01Z
dc.date.available2019-08-29T13:58:01Z
dc.date.issued2018-04-03
dc.identifier.citationTonoyan, Lilit, Boyd, Aoife, Fleming, Gerard T. A., Friel, Ruairi, Gately, Carol M., Mc Cay, Paul H., & O'Flaherty, Vincent. (2018). In vitro comparative cytotoxicity study of a novel biocidal iodo-thiocyanate complex. Toxicology in Vitro, 50, 264-273. doi: https://doi.org/10.1016/j.tiv.2018.03.014en_IE
dc.identifier.issn1879-3177
dc.identifier.urihttp://hdl.handle.net/10379/15362
dc.description.abstractNovel biocides, which avoid the induction of cross-resistance to antibiotics, are an urgent societal requirement. Here, we compared the cytotoxic and bactericidal effects of a new antimicrobial agent, the iodo-thiocyanate complex (ITC), with those of the common antiseptics, hydrogen peroxide (H2O2), povidone iodine (PVP-I) and Lugol's iodine (Lugol). The antimicrobials were co-incubated for 10 min with HeLa and Escherichia coli. cells in the presence and absence of organic matter (Dulbecco's modified Eagle's medium, supplemented with 10% fetal bovine serum). The cytotoxic concentrations of ITC were equivalent to its bactericidal concentrations (7.8 mu g ml(-1)). By contrast, cytotoxic effects of H2O2, PVP-I and Lugol were apparent at concentrations lower than their bactericidal concentrations (250, 250 and 125 mu g ml(-1), respectively). The cellular effects of ITC were not quenched by organic matter, unlike the other antiseptics. ITC, PVP-I and Lugol had hemolytic effect on horse erythrocytes at high concentrations, while H2O2 showed no hemolysis. ITC, at 30 or 300 mu g ml(-1), did not cause DNA breakage in HeLa cells as assessed by an in vitro comet assay in the absence of S9 metabolic activation, whereas H2O2 caused extensive single-strand DNA breaks. The pronounced antimicrobial potency of ITC and its favorable cytotoxicity profile suggests that ITC should be considered for antiseptic applications.en_IE
dc.description.sponsorshipThis work was funded by the Irish Research Council Government of Ireland Postgraduate Scholarship Scheme (GOIPG/2013/584), by Enterprise Ireland (CF/2011/1605) and School of Natural Sciences at National University of Ireland, Galway.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofToxicology In Vitroen
dc.subjectIodo-thiocyanate complex (ITC)en_IE
dc.subjectHydrogen peroxideen_IE
dc.subjectPovidone iodineen_IE
dc.subjectLugol's iodineen_IE
dc.subjectHeLa cytotoxicityen_IE
dc.subjectIn vitro comet assayen_IE
dc.subjectCELL CYTO-TOXICITYen_IE
dc.subjectCOMET ASSAYen_IE
dc.subjectHUMAN-BLOODen_IE
dc.subjectDNA-DAMAGEen_IE
dc.subjectANTISEPTICSen_IE
dc.subjectLACTOPEROXIDASEen_IE
dc.subjectFIBROBLASTSen_IE
dc.subjectMECHANISMSen_IE
dc.subjectSYSTEMen_IE
dc.subjectRANGEen_IE
dc.titleIn vitro comparative cytotoxicity study of a novel biocidal iodo-thiocyanate complexen_IE
dc.typeArticleen_IE
dc.date.updated2019-08-02T09:34:15Z
dc.identifier.doi10.1016/j.tiv.2018.03.014
dc.local.publishedsourcehttps://doi.org/10.1016/j.tiv.2018.03.014en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderIrish Research Councilen_IE
dc.contributor.funderEnterprise Irelanden_IE
dc.contributor.funderSchool of Natural Sciences, National University of Ireland Galway.en_IE
dc.internal.rssid14668948
dc.local.contactVincent O'Flaherty, Dept. Of Microbiology & Eci, Arts/Science Building, Nui Galway. 3734 Email: vincent.oflaherty@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
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