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dc.contributor.authorKeating, Ciara
dc.contributor.authorHughes, D.
dc.contributor.authorMahony, D.
dc.contributor.authorCysneiros, T.
dc.contributor.authorIjaz, U. Z.
dc.contributor.authorSmith, C. J.
dc.contributor.authorO'Flaherty, Vincent
dc.date.accessioned2019-08-29T13:38:14Z
dc.date.available2019-08-29T13:38:14Z
dc.date.issued2018-05-25
dc.identifier.citationKeating, C, Hughes, D, Mahony, T, Cysneiros, D, Ijaz, U Z, Smith, C J, & O'Flaherty, V. (2018). Cold adaptation and replicable microbial community development during long-term low-temperature anaerobic digestion treatment of synthetic sewage. FEMS Microbiology Ecology, 94(7). doi: 10.1093/femsec/fiy095en_IE
dc.identifier.issn1574-6941
dc.identifier.urihttp://hdl.handle.net/10379/15361
dc.description.abstractThe development and activity of a cold-adapting microbial community was monitored during low-temperature anaerobic digestion (LtAD) treatment of wastewater. Two replicate hybrid anaerobic sludge bed-fixed-film reactors treated a synthetic sewage wastewater at 12 degrees C, at organic loading rates of 0.25-1.0 kg chemical oxygen demand (COD) m(-3) d(-1), over 889 days. The inoculum was obtained from a full-scale anaerobic digestion reactor, which was operated at 37 degrees C. Both LtAD reactors readily degraded the influent with COD removal efficiencies regularly exceeding 78% for both the total and soluble COD fractions. The biomass from both reactors was sampled temporally and tested for activity against hydrolytic and methanogenic substrates at 12 degrees C and 37 degrees C. Data indicated that significantly enhanced low-temperature hydrolytic and methanogenic activity developed in both systems. For example, the hydrolysis rate constant (k) at 12 degrees C had increased 20-30-fold by comparison to the inoculum by day 500. Substrate affinity also increased for hydrolytic substrates at low temperature. Next generation sequencing demonstrated that a shift in a community structure occurred over the trial, involving a 1-log-fold change in 25 SEQS (OTU-free approach) from the inoculum. Microbial community structure changes and process performance were replicable in the LtAD reactors.en_IE
dc.description.sponsorshipThis work was supported by Science Foundation Ireland, through a Charles Parsons Award (06_CP_E006) and an Investigator Programme Grant (14/IA/2371); and the Irish Environmental Protection Agency (2014-W-LS-7). CJS is supported by Science Foundation Ireland Starting Investigator-COFUND fellowship (11/SIRG/B2159). UZI is funded by a NERC fellowship NE/L011956/1.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherOxford University Pressen_IE
dc.relation.ispartofFems Microbiology Ecologyen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectanaerobic digestionen_IE
dc.subjectpsychrophilicen_IE
dc.subjecthydrolysisen_IE
dc.subjectmicrobial community structureen_IE
dc.subjectadaptationen_IE
dc.subjectMUNICIPAL WASTE-WATERen_IE
dc.subjectARCHAEAL COMMUNITYen_IE
dc.subjectBIOREACTORen_IE
dc.subjectBACTERIALen_IE
dc.subjectSLUDGEen_IE
dc.subjectDIVERSITYen_IE
dc.subjectREACTORen_IE
dc.subjectBIODEGRADABILITYen_IE
dc.subjectSTABILITYen_IE
dc.subjectOPERATIONen_IE
dc.titleCold adaptation and replicable microbial community development during long-term low-temperature anaerobic digestion treatment of synthetic sewageen_IE
dc.typeArticleen_IE
dc.date.updated2019-08-02T09:38:38Z
dc.identifier.doi10.1093/femsec/fiy095
dc.local.publishedsourcehttps://doi.org/10.1093/femsec/fiy095en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderEnvironmental Protection Agencyen_IE
dc.contributor.funderNatural Environment Research Councilen_IE
dc.internal.rssid14738579
dc.local.contactVincent O'Flaherty, Dept. Of Microbiology & Eci, Arts/Science Building, Nui Galway. 3734 Email: vincent.oflaherty@nuigalway.ie
dc.local.copyrightcheckedYes (APC paid, email 29/08/2019)
dc.local.versionPUBLISHED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/14/IA/2371/IE/i_PAD: Innovative biological phosphate (bioP) and anaerobic digestion (AD) technology for waste treatment, energy generation and phosphorus recovery./en_IE
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/11/SIRG/B2159/IE/Molecular Microbial Ecology of Ammonia Oxidation in Coastal Bay Sediments/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/RCUK/NERC/NE/L011956/1/GB/Undestanding microbial communities through in situ environmental 'omic data synthesis/en_IE
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