Show simple item record

dc.contributor.authorJoyce, Aoife
dc.contributor.authorIjaz, Umer Z.
dc.contributor.authorNzeteu, Corine
dc.contributor.authorVaughan, Aoife
dc.contributor.authorShirran, Sally L.
dc.contributor.authorBotting, Catherine H.
dc.contributor.authorQuince, Christopher
dc.contributor.authorO’Flaherty, Vincent
dc.contributor.authorAbram, Florence
dc.date.accessioned2019-08-29T12:01:19Z
dc.date.available2019-08-29T12:01:19Z
dc.date.issued2018-03-21
dc.identifier.citationJoyce, Aoife, Ijaz, Umer Z., Nzeteu, Corine, Vaughan, Aoife, Shirran, Sally L., Botting, Catherine H., Quince, Christopher, O’Flaherty,Vincent, Abram, Florence. (2018). Linking Microbial Community Structure and Function During the Acidified Anaerobic Digestion of Grass. Frontiers in Microbiology, 9(540). doi: 10.3389/fmicb.2018.00540.en_IE
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10379/15359
dc.description.abstractHarvesting valuable bioproducts from various renewable feedstocks is necessary for the critical development of a sustainable bioeconomy. Anaerobic digestion is a well-established technology for the conversion of wastewater and solid feedstocks to energy with the additional potential for production of process intermediates of high market values (e.g., carboxylates). In recent years, first-generation biofuels typically derived from food crops have been widely utilized as a renewable source of energy. The environmental and socioeconomic limitations of such strategy, however, have led to the development of second-generation biofuels utilizing, amongst other feedstocks, lignocellulosic biomass. In this context, the anaerobic digestion of perennial grass holds great promise for the conversion of sustainable renewable feedstock to energy and other process intermediates. The advancement of this technology however, and its implementation for industrial applications, relies on a greater understanding of the microbiome underpinning the process. To this end, microbial communities recovered from replicated anaerobic bioreactors digesting grass were analyzed. The bioreactors leachates were not buffered and acidic pH (between 5.5 and 6.3) prevailed at the time of sampling as a result of microbial activities. Community composition and transcriptionally active taxa were examined using 16S rRNA sequencing and microbial functions were investigated using metaproteomics. Bioreactor fraction, i.e., grass or leachate, was found to be the main discriminator of community analysis across the three molecular level of investigation (DNA, RNA, and proteins). Six taxa, namely Bacteroidia, Betaproteobacteria, Clostridia, Gammaproteobacteria, Methanomicrobia, and Negativicutes accounted for the large majority of the three datasets. The initial stages of grass hydrolysis were carried out by Bacteroidia, Gammaproteobacteria, and Negativicutes in the grass biofilms, in addition to Clostridia in the bioreactor leachates. Numerous glycolytic enzymes and carbohydrate transporters were detected throughout the bioreactors in addition to proteins involved in butanol and lactate production. Finally, evidence of the prevalence of stressful conditions within the bioreactors and particularly impacting Clostridia was observed in the metaproteomes. Taken together, this study highlights the functional importance of Clostridia during the anaerobic digestion of grass and thus research avenues allowing members of this taxon to thrive should be explored.en_IE
dc.description.sponsorshipThis research was funded by the Irish Higher Education Authority Program for Research in Third Level Institutions Cycle 5: – PRTLI-5 ESI Ph.D. ENS Program. This work was also supported by the Wellcome Trust (grant number 094476/Z/10/Z for the TripleTOF 5600 mass spectrometer at the University of St Andrews), NERC (grant number NE/L011956/1), and a Royal Irish Academy Mobility Grant.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherFrontiers Mediaen_IE
dc.relation.ispartofFrontiers In Microbiologyen
dc.subjectanaerobic digestionen_IE
dc.subjectcellulosic substrateen_IE
dc.subject16S rRNA profilingen_IE
dc.subjectmetaproteomicsen_IE
dc.subjectbiomolecule co-extractionen_IE
dc.subjectWASTE-WATER TREATMENTen_IE
dc.subjectESCHERICHIA-COLIen_IE
dc.subjectSEWAGE-SLUDGEen_IE
dc.subjectBIOGAS PLANTSen_IE
dc.subjectMETAPROTEOMICSen_IE
dc.subjectPROTEINSen_IE
dc.subjectLENGTHen_IE
dc.subjectOPTIMIZATIONen_IE
dc.subjectTEMPERATUREen_IE
dc.subjectPERFORMANCEen_IE
dc.titleLinking microbial community structure and function during the acidified anaerobic digestion of grassen_IE
dc.typeArticleen_IE
dc.date.updated2019-08-02T09:42:07Z
dc.identifier.doi10.3389/fmicb.2018.00540
dc.local.publishedsourcehttps://doi.org/10.3389/fmicb.2018.00540en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderWellcome Trusten_IE
dc.contributor.funderNatural Environment Research Councilen_IE
dc.internal.rssid14150423
dc.local.contactVincent O'Flaherty, Dept. Of Microbiology & Eci, Arts/Science Building, Nui Galway. 3734 Email: vincent.oflaherty@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
nui.item.downloads17


Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:

Thumbnail

This item appears in the following Collection(s)

Show simple item record