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dc.contributor.authorTonoyan, Lilit
dc.contributor.authorFleming, Gerard T. A.
dc.contributor.authorFriel, Ruairi
dc.contributor.authorO’Flaherty, Vincent
dc.date.accessioned2019-08-27T15:18:38Z
dc.date.available2019-08-27T15:18:38Z
dc.date.issued2019-02-20
dc.identifier.citationTonoyan, Lilit, Fleming, Gerard T. A., Friel, Ruairi, & O’Flaherty, Vincent. (2019). Continuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance. Scientific Reports, 9(1), 2401. doi: 10.1038/s41598-019-38925-9en_IE
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10379/15354
dc.description.abstractWe attempted to generate de novo resistance to a newly described biocidal complex, ITC (iodo-thiocyanate complex), and to levofloxacin (LVX) in Escherichia coli ATCC 25922, by means of selective chemostat culture. We measured resistance by determining the minimum inhibitory concentrations (MICs) for these agents. E. coli underwent 20-day parallel adaptive evolution routes under no antimicrobial selection, and gradually increasing ITC and LVX selection pressure. Long-term exposure of E. coli to ITC did not induce resistance to ITC, or cross-resistance to LVX. No distinct mutational pattern was evidenced from whole-genome sequence (WGS)-based comparisons of ITC-challenged and unchallenged bacterial populations. Moreover, the exposed E. coli population could not survive a 2 x MIC challenge of ITC. By contrast, resistance to LVX was rapidly induced (on day 1 the MIC had increased 16-fold), selected for (by day 14 the MIC had increased 64-fold) and enriched with a highly characteristic genome mutational pattern. WGS of this evolving population revealed that the majority of mutations appeared in the genes of LVX target proteins (GyrA, ParC, ParE) and drug influx (OmpF). This study suggests that the usage of ITC may not trigger the emergence of facile resistance or cross-resistance, in contrast to common antibiotics.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherNature Researchen_IE
dc.relation.ispartofScientific Reportsen
dc.subjectDNA MISMATCH REPAIRen_IE
dc.subjectANTIBIOTIC-RESISTANCEen_IE
dc.subjectSTAPHYLOCOCCUS-AUREUSen_IE
dc.subjectIN-VITROen_IE
dc.subjectMECHANISMSen_IE
dc.subjectEVOLUTIONen_IE
dc.subjectDISINFECTANTSen_IE
dc.subjectLEVOFLOXACINen_IE
dc.subjectBIOCIDEen_IE
dc.subjectSUSCEPTIBILITYen_IE
dc.titleContinuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistanceen_IE
dc.typeArticleen_IE
dc.date.updated2019-08-01T07:47:19Z
dc.identifier.doi10.1038/s41598-019-38925-9
dc.local.publishedsourcehttps://dx.doi.org/10.1038/s41598-019-38925-9en_IE
dc.description.peer-reviewedpeer-reviewed
dc.internal.rssid15952250
dc.local.contactVincent O'Flaherty, Dept. Of Microbiology & Eci, Arts/Science Building, Nui Galway. 3734 Email: vincent.oflaherty@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
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