Show simple item record

dc.contributor.authorDonnelly, Eleanor M.
dc.contributor.authorStrappe, Padraig M.
dc.contributor.authorMcGinley, Lisa M.
dc.contributor.authorMadigan, Nicolas N.
dc.contributor.authorGeurts, Elizabeth
dc.contributor.authorRooney, Gemma E.
dc.contributor.authorWindebank, Anthony J.
dc.contributor.authorFraher, John
dc.contributor.authorDockery, Peter
dc.contributor.authorO'Brien, Timothy
dc.contributor.authorMcMahon, Siobhan S.
dc.date.accessioned2019-08-27T10:22:14Z
dc.date.available2019-08-27T10:22:14Z
dc.date.issued2010
dc.identifier.citationDonnelly, Eleanor M., Strappe, Padraig M., McGinley, Lisa M., Madigan, Nicolas N., Geurts, Elizabeth, Rooney, Gemma E., Windebank, Anthony J., Fraher, John, Dockery, Peter, O'Brien, Timothy, McMahon, Siobhan S. (2010). Lentiviral vector-mediated knockdown of the neuroglycan 2 proteoglycan or expression of neurotrophin-3 promotes neurite outgrowth in a cell culture model of the glial scar. The Journal of Gene Medicine, 12(11), 863-872. doi: 10.1002/jgm.1509en_IE
dc.identifier.issn1521-2254
dc.identifier.urihttp://hdl.handle.net/10379/15349
dc.description.abstractBackground Following spinal cord injury, a highly inhibitory environment for axonal regeneration develops. One of the main sources of this inhibition is the glial scar that is formed after injury by reactive astrocytes. The inhibitory environment is mainly a result of chondroitin sulphate proteoglycans (CSPGs). Neuroglycan 2 (NG2), one of the main inhibitory CSPGs, is up-regulated following spinal cord injury.Methods Small interfering RNA (siRNA) was designed to target NG2 and this short hairpin RNA (shRNA) was cloned into a lentiviral vector (LV). The neurotrophic factor neurotrophin-3 (NT-3) promotes the growth and survival of developing neurites and has also been shown to aid regeneration. NT-3 was also cloned into a LV. In vitro assessment of these vectors using a coculture system of dorsal root ganglia (DRG) neurones and Neu7 astrocytes was carried out. The Neu7 cell line is a rat astrocyte cell line that overexpresses NG2, thereby mimicking the inhibitory environment following spinal cord injury.Results and Discussion These experiments show that both the knockdown of NG2 via shRNA and over-expression of NT-3 can significantly increase neurite growth, although a combination of both vectors did not confer any additional benefit over the vectors used individually. These LVs show promising potential for growth and survival of neurites in injured central nervous system tissue (CNS). Copyright (C) 2010 John Wiley & Sons, Ltd.en_IE
dc.description.sponsorshipWe wish to thank Professor Dider Trono (EFPL, Switzerland) for the kind gift of the lentiviral vector gene transfer and packaging plasmids and Dr John Rogers and Professor James Fawcett (University of Cambridge) for their kind gift of the Neu7 astrocyte cell line. This work was supported by funding from the Health Research Board of Ireland and Science Foundation Ireland.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherWileyen_IE
dc.relation.ispartofJournal Of Gene Medicineen
dc.subjectglial scaren_IE
dc.subjectlentiviral vectoren_IE
dc.subjectNeu7 astrocytesen_IE
dc.subjectneurotrophin-3en_IE
dc.subjectNG2en_IE
dc.subjectspinal cord injuryen_IE
dc.subjectSPINAL-CORD-INJURYen_IE
dc.subjectCHONDROITIN SULFATE PROTEOGLYCANen_IE
dc.subjectOLFACTORY ENSHEATHING GLIAen_IE
dc.subjectAXON GROWTHen_IE
dc.subjectGENE-TRANSFERen_IE
dc.subjectHINDLIMB FUNCTIONen_IE
dc.subjectREGENERATIONen_IE
dc.subjectNG2en_IE
dc.subjectRECOVERYen_IE
dc.subjectINHIBITIONen_IE
dc.titleLentiviral vector‐mediated knockdown of the neuroglycan 2 proteoglycan or expression of neurotrophin‐3 promotes neurite outgrowth in a cell culture model of the glial scaren_IE
dc.typeArticleen_IE
dc.date.updated2014-02-24T22:31:19Z
dc.identifier.doi10.1002/jgm.1509
dc.local.publishedsourcehttps://doi.org/10.1002/jgm.1509en_IE
dc.description.peer-reviewedpeer-reviewed
dc.internal.rssid1335832
dc.local.contactSiobhan Mcmahon, Department Of Anatomy, Nui, Galway. 2838 Email: siobhan.mcmahon@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
nui.item.downloads170


Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:

Thumbnail

This item appears in the following Collection(s)

Show simple item record