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dc.contributor.authorMcMahon, Siobhan S.
dc.contributor.authorAlbermann, Silke
dc.contributor.authorRooney, Gemma E.
dc.contributor.authorShaw, Georgina
dc.contributor.authorGarcia, Yolanda
dc.contributor.authorSweeney, Eva
dc.contributor.authorHynes, Jacqueline
dc.contributor.authorDockery, Peter
dc.contributor.authorO'Brien, Timothy
dc.contributor.authorWindebank, Anthony J.
dc.contributor.authorAllsopp, Timothy E.
dc.contributor.authorBarry, Frank P.
dc.date.accessioned2019-08-26T12:55:37Z
dc.date.available2019-08-26T12:55:37Z
dc.date.issued2012-12-20
dc.identifier.citationMcMahon, Siobhan S., Albermann, Silke, Rooney, Gemma E., Shaw, Georgina, Garcia, Yolanda, Sweeney, Eva, Hynes, Jacqueline, Dockery, Peter, O'Brien, Timothy, Windebank, Anthony J., Allsopp, Timothy E., Barry, Frank P. (2010). Engraftment, migration and differentiation of neural stem cells in the rat spinal cord following contusion injury. Cytotherapy, 12(3), 313-325. doi: https://doi.org/10.3109/14653241003695018en_IE
dc.identifier.issn1477-2566
dc.identifier.urihttp://hdl.handle.net/10379/15346
dc.description.abstractBackground aims. Spinal cord injury is a devastating injury that impacts drastically on the victim's quality of life. Stem cells have been proposed as a therapeutic strategy. Neural stem (NS) cells have been harvested from embryonic mouse forebrain and cultured as adherent cells. These NS cells express markers of neurogenic radial glia. Methods. Mouse NS cells expressing green fluorescent protein (GFP) were transplanted into immunosupressed rat spinal cords following moderate contusion injury at T9. Animals were left for 2 and 6 weeks then spinal cords were fixed, cryosectioned and analyzed. Stereologic methods were used to estimate the volume and cellular environment of the lesions. Engraftment, migration and differentiation of NS cells were also examined. Results. NS cells integrated well into host tissue and appeared to migrate toward the lesion site. They expressed markers of neurons, astrocytes and oligodendrocytes at 2 weeks post-transplantation and markers of neurons and astrocytes at the 6-week time-point. NS cells appeared to have a similar morphologic phenotype to radial glia, in particular at the pial surface. Conclusions. Although no functional recovery was observed using the Basso Beattie Bresnahan (BBB) locomotor rating scale, NS cells are a potential cellular therapy for treatment of injured spinal cord. They may be used as delivery vehicles for therapeutic proteins because they show an ability to migrate toward the site of a lesion. They may also be used to replace lost or damaged neurons and oligodendrocytes.en_IE
dc.description.sponsorshipThis research was funded by Science Foundation Ireland. The authors would like to thank Dr Ariella Magee and Eleanor Donnelly for technical assistance.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofCytotherapyen
dc.subjectradial gliaen_IE
dc.subjectregenerationen_IE
dc.subjectspinal cord injuryen_IE
dc.subjectstereologyen_IE
dc.subjecttransplantationen_IE
dc.subjectPROMOTE FUNCTIONAL RECOVERYen_IE
dc.subjectPROGENITOR CELLSen_IE
dc.subjectADULT-RATen_IE
dc.subjectORGANOTYPIC COCULTURESen_IE
dc.subjectNEUROTROPHIC FACTORSen_IE
dc.subjectAXON GROWTHen_IE
dc.subjectTRANSPLANTATIONen_IE
dc.subjectMICEen_IE
dc.subjectREPAIRen_IE
dc.subjectPROLIFERATIONen_IE
dc.titleEngraftment, migration and differentiation of neural stem cells in the rat spinal cord following contusion injuryen_IE
dc.typeArticleen_IE
dc.date.updated2014-02-24T22:33:57Z
dc.identifier.doi10.3109/14653241003695018
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.internal.rssid1338040
dc.local.contactSiobhan Mcmahon, Department Of Anatomy, Nui, Galway. 2838 Email: siobhan.mcmahon@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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